Lima Mariana Dos Reis, Lopes Amanda P, Martins Conceição, Brito Gerly A C, Carneiro Virgínia C, Goes Paula
Nucleus of Study and Research in Pain, Inflammation, and Osteoimmunology, Department of Morphology, Medical School, Federal University of CearáFortaleza, Brazil.
Nucleus of Study and Research in Pain, Inflammation, and Osteoimmunology, Department of Pathology and Legal Medicine, Medical School, Federal University of CearáFortaleza, Brazil.
Front Physiol. 2017 Jun 28;8:440. doi: 10.3389/fphys.2017.00440. eCollection 2017.
Periodontitis is associated with reduced antioxidant capacity and increased oxidative damage. Oxidative stress induces inflammation and bone loss contributing to the pathological progression of periodontal disease. (CLO) has demonstrated anti-inflammatory and anti-oxidant activities. Therefore, the aim of this study was to evaluate the effect of CLO on oxidative stress and bone loss in rats subjected to experimental periodontitis (EP). For this, 72 male Wistar rats were divided into groups: Naïve, Saline (SAL) and CLO. Rats received SAL or CLO (90 mg/kg) 30 min before ligature and daily until the 11th day. Naïve group experienced no manipulation. After 11 days, the animals were euthanized and left maxillae collected for macroscopic analysis of alveolar bone loss (ABL). Periodontium was analyzed by macroscopy, scanning electron microscopy; confocal and light polarized microscopy. Immunohistochemical examination of DKK1, WNT 10b and β-catenin was performed. The gingival tissue was collected to reduced glutathione (GSH), superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) analyses. The 11 days of ligature induced bone loss, breakdown of collagen fibers, increased the immunostaining DKK-1 while reduced WNT 10b and β-catenin expressions. Periodontitis reduced GSH, SOD, CAT and increase MDA. All findings were reversed by 90 mg/kg of CLO. In summary our findings demonstrated that CLO reduced oxidative stress and bone loss and preserved collagen fibers in rats with EP, with participation of WNT signaling pathway.
牙周炎与抗氧化能力降低和氧化损伤增加有关。氧化应激会引发炎症和骨质流失，促进牙周疾病的病理进展。（某种物质，未明确，暂用CLO指代）已显示出抗炎和抗氧化活性。因此，本研究的目的是评估CLO对实验性牙周炎（EP）大鼠氧化应激和骨质流失的影响。为此，将72只雄性Wistar大鼠分为几组：未处理组、生理盐水组（SAL）和CLO组。大鼠在结扎前30分钟接受SAL或CLO（90毫克/千克），并每天给药直至第11天。未处理组不进行任何操作。11天后，对动物实施安乐死并收集左侧上颌骨，用于牙槽骨丢失（ABL）的宏观分析。通过宏观检查、扫描电子显微镜、共聚焦显微镜和光偏振显微镜对牙周组织进行分析。对DKK1、WNT 10b和β-连环蛋白进行免疫组织化学检查。收集牙龈组织进行还原型谷胱甘肽（GSH）、超氧化物歧化酶（SOD）、过氧化氢酶（CAT）和丙二醛（MDA）分析。结扎11天导致骨质流失、胶原纤维断裂，DKK-1免疫染色增加，而WNT 10b和β-连环蛋白表达降低。牙周炎使GSH、SOD、CAT降低，MDA增加。所有这些结果都被90毫克/千克的CLO逆转。总之，我们的研究结果表明，CLO可减轻EP大鼠的氧化应激和骨质流失，并保留胶原纤维，这与WNT信号通路有关。
