Graciet Emmanuelle, Lebreton Sandrine, Gontero Brigitte
Laboratoire d'ingéniérie des protéines et contrôle métabolique, Département Biologie des génomes, Institut Jacques Monod, UMR 7592 CNRS, Universités Paris VI-VII, 2 place Jussieu, F-75251 Paris cedex 05, France.
J Exp Bot. 2004 May;55(400):1245-54. doi: 10.1093/jxb/erh107. Epub 2004 Mar 26.
Protein-protein interactions are involved in many metabolic pathways. This review will focus on the role of such associations in CO2 assimilation (Benson-Calvin cycle) and especially on the involvement of a GAPDH/CP12/PRK complex which has been identified in many photosynthetic organisms and may have an important role in the regulation of CO2 assimilation. The emergence of new kinetic and regulatory properties as a consequence of protein-protein interactions will be addressed as well as some of the questions raised by the existence of these supramolecular complexes such as composition, function, and assembly pathways. The presence and role of small intrinsically unstructured proteins like the 8.5 kDa protein CP12, involved in the regulation and/or assembly of these complexes will be discussed.
蛋白质-蛋白质相互作用参与许多代谢途径。本综述将聚焦于此类相互作用在二氧化碳同化（卡尔文循环）中的作用，尤其关注已在许多光合生物中鉴定出的甘油醛-3-磷酸脱氢酶/CP12/磷酸核酮糖激酶复合物的参与情况，该复合物可能在二氧化碳同化的调节中发挥重要作用。将探讨因蛋白质-蛋白质相互作用而产生的新动力学和调节特性，以及这些超分子复合物的存在所引发的一些问题，如组成、功能和组装途径。还将讨论像8.5 kDa的CP12蛋白这类参与这些复合物调节和/或组装的小内在无序蛋白的存在及作用。
