Ciprandi Giorgio, Cirillo Ignazio, Vizzaccaro Andrea, Milanese Manlio, Tosca Maria Angela
Ospedale San Martino, Genoa, Italy.
Int Arch Allergy Immunol. 2004 May;134(1):34-40. doi: 10.1159/000077531. Epub 2004 Mar 25.
Nasal obstruction is a typical symptom of allergic rhinitis. Allergic rhinitis is characterized by a Th2-dependent inflammation. The aim of this study was to evaluate the possible relationships between allergic inflammation, including inflammatory cells and cytokine pattern, and nasal airflow in patients with nasal obstruction due to seasonal allergic rhinitis.
Fifty patients (31 males and 19 females, mean age 31.9 +/- 4.8 years) with seasonal allergic rhinitis were evaluated during the pollen season. All of them had moderate to severe nasal obstruction. Total symptom score, rhinomanometry, nasal lavage, and nasal scraping were assessed in all subjects. Inflammatory cells were counted by conventional staining; IL-4, IL-5, IL-8, and IFNgamma were measured by immunoassay on fluids recovered from nasal lavage.
Significant positive relationships were demonstrated between eosinophil infiltration and IL-4 levels (p < 0.0001), eosinophils and IL-5 levels (p < 0.0001), and eosinophils and IL-8 levels (p < 0.0001). Significant negative relationships were demonstrated between eosinophil infiltration and IFNgamma levels (p < 0.0001) and eosinophil infiltration and nasal airflow (p < 0.001).
This study demonstrates the close connection between Th2 cytokines and eosinophil infiltration. In addition, there is clear evidence concerning the relationship between eosinophil infiltration and nasal airflow. These findings constitute first evidence of the relationship between nasal airflow impairment and Th2-related inflammation in seasonal allergic rhinitis.
鼻塞是变应性鼻炎的典型症状。变应性鼻炎以Th2依赖性炎症为特征。本研究的目的是评估季节性变应性鼻炎所致鼻塞患者的变应性炎症（包括炎症细胞和细胞因子模式）与鼻气流之间的可能关系。
在花粉季节对50例季节性变应性鼻炎患者（31例男性和19例女性，平均年龄31.9±4.8岁）进行评估。所有患者均有中度至重度鼻塞。对所有受试者评估总症状评分、鼻阻力测量、鼻腔灌洗和鼻刮片检查。通过传统染色计数炎症细胞；通过免疫测定法检测从鼻腔灌洗液中回收的液体中的IL-4、IL-5、IL-8和IFNγ。
嗜酸性粒细胞浸润与IL-4水平之间（p<0.0001）、嗜酸性粒细胞与IL-5水平之间（p<0.0001）以及嗜酸性粒细胞与IL-8水平之间（p<0.0001）显示出显著的正相关关系。嗜酸性粒细胞浸润与IFNγ水平之间（p<0.0001）以及嗜酸性粒细胞浸润与鼻气流之间（p<0.001）显示出显著的负相关关系。
本研究证明了Th2细胞因子与嗜酸性粒细胞浸润之间的密切联系。此外，有明确证据表明嗜酸性粒细胞浸润与鼻气流之间的关系。这些发现构成了季节性变应性鼻炎中鼻气流受损与Th2相关炎症之间关系的首个证据。
