Zhao Yu-Qing, Liu Xiao-Hong, Ito Tetsuhide, Qian Jia-Ming
Department of Gastroenterology, Peking Union Medical College Hospital, Peking Union Medical College and Chinese Academy of Medical Science, Beijing 100730, China.
World J Gastroenterol. 2004 Apr 1;10(7):1005-9. doi: 10.3748/wjg.v10.i7.1005.
To investigate the effects of rhubarb on severe acute pancreatitis (SAP) in rats.
Severe acute pancreatitis was induced by two intraperitoneal injections of cerulein (40 microg/kg body weight) plus 5-h restraint water-immersion stress. Rhubarb (75-150 mg/kg) was orally fed before the first cerulein injection. The degree of pancreatic edema, serum amylase level, local pancreatic blood flow (PBF), and histological alterations were investigated. The effects of rhubarb on pancreatic exocrine secretion in this model were evaluated by comparing with those of somatostatin.
In the Cerulein+Stress group, severe edema and diffuse hemorrhage in the pancreas were observed, the pancreatic wet weight (11.60+/-0.61 g/Kg) and serum amylase (458 490+/-43 100 U/L) were markedly increased (P<0.01 vs control). In the rhubarb (150 mg/kg) treated rats, necrosis and polymorphonuclear neutrophil (PMN) infiltration in the pancreas were significantly reduced (P<0.01), and a marked decrease (50%) in serum amylase levels was also observed (P<0.01). PBF dropped to 38% (93+/-5 mL/min per 100 g) of the control in the Cerulein+Stress group and partly recovered in the Cerulein+Stress+Rhubarb 150 mg group (135+/-12 mL/min per 100 g) (P<0.01). The pancreatic exocrine function was impaired in the SAP rats. The amylase levels of pancreatic juice were reduced in the rats treated with rhubarb or somatostatin, comparing with that of untreated SAP group. The bicarbonate concentration of pancreatic juice was markedly elevated only in the rhubarb-treated group (P<0.01).
Rhubarb can exert protective effects on SAP, probably by inhibiting the inflammation of pancreas, improving pancreatic microcirculation, and altering exocrine secretion.
研究大黄对大鼠重症急性胰腺炎（SAP）的影响。
通过两次腹腔注射雨蛙素（40微克/千克体重）加5小时束缚水浸应激诱导重症急性胰腺炎。在首次注射雨蛙素前口服给予大黄（75 - 150毫克/千克）。研究胰腺水肿程度、血清淀粉酶水平、局部胰腺血流（PBF）及组织学改变。通过与生长抑素比较评估大黄在此模型中对胰腺外分泌的影响。
在雨蛙素+应激组，观察到胰腺严重水肿和弥漫性出血，胰腺湿重（11.60±0.61克/千克）和血清淀粉酶（458490±43100单位/升）显著升高（与对照组相比，P<0.01）。在大黄（150毫克/千克）治疗的大鼠中，胰腺坏死和多形核中性粒细胞（PMN）浸润显著减少（P<0.01），血清淀粉酶水平也显著降低（50%）（P<0.01）。在雨蛙素+应激组，PBF降至对照组的38%（93±5毫升/分钟/100克），在雨蛙素+应激+大黄150毫克组部分恢复（135±12毫升/分钟/100克）（P<0.01）。SAP大鼠的胰腺外分泌功能受损。与未治疗的SAP组相比，大黄或生长抑素治疗的大鼠胰液淀粉酶水平降低。仅大黄治疗组胰液碳酸氢盐浓度显著升高（P<0.01）。
大黄可对SAP发挥保护作用，可能是通过抑制胰腺炎症、改善胰腺微循环及改变外分泌实现的。
