银杏叶提取物对老年大鼠肝脏的影响。
Effect of ginkgo biloba extract on livers in aged rats.
作者信息
Huang Shang-Zhen, Luo Yan-Jun, Wang Li, Cai Ke-Yin
机构信息
Department of Geriatrics, Wuhan General Hospital, Guangzhou Command of PLA, Wuhan, Hubei Province, China.
出版信息
World J Gastroenterol. 2005 Jan 7;11(1):132-5. doi: 10.3748/wjg.v11.i1.132.
AIM
To investigate the protective effect of ginkgo biloba extract (GBE) on livers of aged rats and the associated mechanisms.
METHODS
Two-mo- and 20-mo-old rats were treated with GBE/saline for 3 mo. Liver tissue samples from 5-mo-old rats treated with saline (group Y) and 23-mo-old rats treated with GBE (group E) or saline (group N) were used for histopathological examinations (hematoxylin-eosin and Masson staining, Lipofuscin staining-Schmorl staining) and determination of expression of tissue inhibitor-1 of metalloproteinase (TIMP-1) and the level of malondialdehyde (MDA), glutathione peroxidase (GPx) and superoxide dismutase (SOD). Blood samples were collected for determination of alanine aminotransferase (ALT), aspartate aminotransferase (AST), total bilirubin (TBIL) and albumin.
RESULTS
Microscopic studies with Masson staining revealed mild liver fibrosis in aged rats (group N), while the livers of aged rats receiving GBE (group E) showed amelioration in fibrosis (2.2+/-0.1 vs 2.8+/-0.1, P<0.01) and deposition of lipofuscin (33.7+/-5.3 vs 62.8+/-5.7, P<0.01). The expression of TIMP-1 and the level of liver MDA (1.0+/-0.1 vs 1.2+/-0.2, P<0.05) also decreased but the activity of GPx (97.1+/-15.3 vs 61.8+/-14.5, P<0.01) increased in group E. Compared with group Y, the level of liver MDA (0.8+/-0.1 vs 1.2+/-0.2, P<0.01), lipofuscin (32.4+/-6.0 vs 62.8+/-5.7, P<0.01) and TIMP-1 expression were increased, while the activity of GPx (103.2+/-17.6 vs 61.8+/-14.5, P<0.01) and SOD (16.7+/-4.4 vs 11.8+/-3.9, P<0.05) was decreased in group N. There was no difference in liver function among these three groups.
CONCLUSION
GBE has protective effects on aging liver. The possible mechanisms might be its antioxidant activity and inhibition of TIMP-1 expression.
目的
研究银杏叶提取物（GBE）对老龄大鼠肝脏的保护作用及其相关机制。
方法
将2月龄和20月龄大鼠分别用GBE/生理盐水处理3个月。取生理盐水处理的5月龄大鼠（Y组）、GBE处理的23月龄大鼠（E组）和生理盐水处理的23月龄大鼠（N组）的肝脏组织样本进行组织病理学检查（苏木精-伊红染色、Masson染色、脂褐素染色-Schmorl染色），并测定金属蛋白酶组织抑制剂-1（TIMP-1）的表达、丙二醛（MDA）水平、谷胱甘肽过氧化物酶（GPx）和超氧化物歧化酶（SOD）活性。采集血液样本测定谷丙转氨酶（ALT）、谷草转氨酶（AST）、总胆红素（TBIL）和白蛋白水平。
结果
Masson染色显微镜检查显示老龄大鼠（N组）有轻度肝纤维化，而接受GBE治疗的老龄大鼠（E组）肝脏纤维化有所改善（2.2±0.1对2.8±0.1，P<0.01），脂褐素沉积减少（33.7±5.3对62.8±5.7，P<0.01）。E组TIMP-1表达和肝脏MDA水平也降低（1.0±0.1对1.2±0.2，P<0.05），但GPx活性增加（97.1±15.3对61.8±14.5，P<0.01）。与Y组相比，N组肝脏MDA水平（0.8±0.1对1.2±0.2，P<0.01）、脂褐素（32.4±6.0对62.8±5.7，P<0.01）和TIMP-1表达增加，而GPx活性（103.2±17.6对61.8±14.5，P<0.01）和SOD活性（16.7±4.4对11.8±3.9，P<0.05）降低。三组肝功能无差异。
结论
GBE对老龄肝脏具有保护作用。其可能机制可能是抗氧化活性和抑制TIMP-1表达。
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