Pavlov Valeri, Xiao Yi, Gill Ron, Dishon Arnon, Kotler Moshe, Willner Itamar
The Institute of Chemistry, The Farkas Center for Light-Induced Processes, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Anal Chem. 2004 Apr 1;76(7):2152-6. doi: 10.1021/ac035219l.
A G-rich nucleic acid sequence binds hemin and yields a biocatalytic complex (DNAzyme) of peroxidase activity, namely, the biocatalyzed generation of chemiluminescence in the presence of H(2)O(2) and luminol. The DNAzyme is used as a label for the amplified detection of DNA, or for the analysis of telomerase activity in cancer cells, using chemiluminescence as an output signal. In one configuration, the analyzed DNA is hybridized with a primer nucleic acid that is associated with a Au surface, and the DNAzyme label is hybridized with the surface-confined analyte DNA. The DNA is analyzed with a detection limit of approximately 1 x 10(-)(9) M. In the second system, telomerase from HeLa cancer cells induces telomerization of a primer associated with a Au surface and the complementary DNAzyme units are hybridized with the telomere to yield the chemiluminescence. The detection limit of the system corresponds to 1000 HeLa cells in the analyzed sample.
富含鸟嘌呤的核酸序列与血红素结合，产生具有过氧化物酶活性的生物催化复合物（脱氧核酶），即在过氧化氢和鲁米诺存在的情况下生物催化产生化学发光。脱氧核酶用作DNA扩增检测的标记，或用于癌细胞中端粒酶活性的分析，以化学发光作为输出信号。在一种配置中，被分析的DNA与与金表面相关的引物核酸杂交，并且脱氧核酶标记与表面受限的分析物DNA杂交。分析DNA的检测限约为1×10⁻⁹ M。在第二个系统中，来自HeLa癌细胞的端粒酶诱导与金表面相关的引物的端粒化，并且互补的脱氧核酶单元与端粒杂交以产生化学发光。该系统的检测限对应于分析样品中的1000个HeLa细胞。
