The Institute of Chemistry, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
The Institute of Life Science, The Hebrew University of Jerusalem, Jerusalem 91904, Israel.
Nano Lett. 2023 Sep 27;23(18):8664-8673. doi: 10.1021/acs.nanolett.3c02542. Epub 2023 Sep 5.
Glucose oxidase-loaded ZIF-90 metal-organic framework nanoparticles conjugated to hemin-G-quadruplexes act as functional bioreactor hybrids operating transient dissipative biocatalytic cascaded transformations consisting of the glucose-driven HO-mediated oxidation of Amplex-Red to resorufin or the glucose-driven generation of chemiluminescence by the HO-mediated oxidation of luminol. One system involves the fueled activation of a reaction module leading to the temporal formation and depletion of the bioreactor conjugate operating the nickase-guided transient biocatalytic cascades. The second system demonstrates the fueled activation of a reaction module yielding a bioreactor conjugate operating the exonuclease III-dictated transient operation of the two biocatalytic cascades. The temporal operations of the bioreactor circuits are accompanied by kinetic models and computational simulations enabling us to predict the dynamic behavior of the systems subjected to different auxiliary conditions.
载有葡萄糖氧化酶的 ZIF-90 金属有机骨架纳米粒子与血红素-G-四链体连接，作为功能生物反应器杂合体，进行瞬态耗散生物催化级联转化，包括葡萄糖驱动的 HO 介导的 Amplex-Red 向 Resorufin 的氧化或葡萄糖驱动的 HO 介导的 Luminol 氧化产生化学发光。一个系统涉及反应模块的燃料激活，导致生物反应器偶联物的时空形成和耗尽，该偶联物操作尼克酰胺引导的瞬态生物催化级联。第二个系统证明了反应模块的燃料激活产生生物反应器偶联物，该偶联物操作外切酶 III 指示的两个生物催化级联的瞬态操作。生物反应器电路的时间操作伴随着动力学模型和计算模拟，使我们能够预测在不同辅助条件下系统的动态行为。
