从四种蘑菇的废弃堆肥中检测和回收水解酶
Detection and recovery of hydrolytic enzymes from spent compost of four mushroom species.
作者信息
Ko Han Gyu, Park Sang Ho, Kim Seong Hwan, Park Hyuk Gu, Park Won Mok
机构信息
School of Life Sciences and Biotechnology, Korea University, Seoul 136-701, Korea.
出版信息
Folia Microbiol (Praha). 2005;50(2):103-6. doi: 10.1007/BF02931456.
To evaluate the potential of using the enzymes from spent mushroom compost (SMC) as an industrial enzyme, the production of alpha-amylase, cellulase, beta-glucosidase, laccase, and xylanase was determined from the SMC of four edible mushroom species (Pleurotus ostreatus, Lentinula edodes, Flammulina velutipes and Hericium erinaceum). Among the tested SMC, the SMC of L. edodes showed the highest enzyme activity in alpha-amylase (229 nkat/g), cellulase (759 nkat/g) and beta-glucosidase (767 nkat/g) in 0.5% Triton X-100, and that of P. ostreatus showed the highest activity in laccase (1452 nkat/g) in phosphate-buffered 0.2% Triton X-100. The highest xylanase activity (119 nkat/g) was found in the SMC of F. velutipes.
为了评估利用废弃蘑菇培养料（SMC）中的酶作为工业酶的潜力，测定了四种食用菌（平菇、香菇、金针菇和猴头菇）的SMC中α-淀粉酶、纤维素酶、β-葡萄糖苷酶、漆酶和木聚糖酶的产量。在所测试的SMC中，香菇的SMC在0.5% Triton X-100中，α-淀粉酶（229 nkat/g）、纤维素酶（759 nkat/g）和β-葡萄糖苷酶（767 nkat/g）的酶活性最高；平菇的SMC在磷酸盐缓冲的0.2% Triton X-100中，漆酶（1452 nkat/g）的活性最高。金针菇的SMC中木聚糖酶活性最高（119 nkat/g）。
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