一株粉红色兼性甲基营养菌甲基杆菌属MB1的生长及酶学特性

Growth and enzymological characteristics of a pink-pigmented facultative methylotroph Methylobacterium sp. MB1.

作者信息

Baev M V, Kuznetsov E V, Skladnev D A, Govorukhina N I, Sterkin V E, Tsygankov Y D

机构信息

Institute of Genetics and Selection of Industrial Microorganisms, Moscow, Russian Federation.

出版信息

Folia Microbiol (Praha). 1992;37(2):93-101. doi: 10.1007/BF02836611.

DOI:10.1007/BF02836611

PMID:1505877

Abstract

Growth characteristics of batch and continuous cultures of the pink facultative methylotroph Methylobacterium sp. MB1 were determined. The response of a chemostat culture to a pulse increase of methanol concentration was studied. Malate, succinate and oxaloacetate additions to the methanol-supplemented medium decreased batch culture growth inhibition by methanol. The carotenoid content in cells grown in a chemostat decreased with increasing growth rate. The key enzyme activities of C1-metabolism were measured in a chemostat culture at different dilution rates.

摘要

测定了兼性甲基营养菌粉红甲基杆菌MB1分批培养和连续培养的生长特性。研究了恒化器培养对甲醇浓度脉冲增加的响应。向添加甲醇的培养基中添加苹果酸、琥珀酸和草酰乙酸可降低甲醇对分批培养生长的抑制作用。在恒化器中生长的细胞中类胡萝卜素含量随生长速率的增加而降低。在不同稀释率下，在恒化器培养中测定了C1代谢的关键酶活性。