血管内皮生长因子-634G/C和936C/T基因多态性与自发性早产的相关性
Association of -634G/C and 936C/T polymorphisms of the vascular endothelial growth factor with spontaneous preterm delivery.
作者信息
Papazoglou Dimitrios, Galazios Georgios, Koukourakis Michael I, Kontomanolis Emmanuel N, Maltezos Efstratios
机构信息
Department of Internal Medicine, Medical School, Democritus University of Thrace, Patriarhou Grigoriou 97-99, 68100 Alexandroupolis, Greece.
出版信息
Acta Obstet Gynecol Scand. 2004 May;83(5):461-5. doi: 10.1111/j.0001-6349.2004.00403.x.
BACKGROUND
There is convincing evidence for a central role of vascular endothelial growth factor (VEGF) in fetal and placental angiogenesis. Our present study was undertaken to examine the possible relationship between two common functional VEGF gene polymorphisms (- 634G/C and 936C/T), linked with altered VEGF gene responsiveness, and spontaneous preterm delivery.
METHODS
Genomic DNA was extracted from whole blood from 54 women with preterm labor and 79 menopausal women with at least two term spontaneous labors. DNA samples were analyzed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP).
RESULTS
Individuals with 936T/T or 936C/T genotype demonstrated a statistically significant association with preterm delivery compared with those sharing 936C/C genotype [P = 0.0009, risk factor 2.05, 95% confidence interval (CI) 1.37-3.06]. There were no significant associations between spontaneous preterm delivery and - 634 genotypes.
CONCLUSION
An association was demonstrated between the VEGF 936C/T polymorphism and deliveries before 37 weeks of gestation.
背景
有令人信服的证据表明血管内皮生长因子（VEGF）在胎儿和胎盘血管生成中起核心作用。我们目前的研究旨在探讨与VEGF基因反应性改变相关的两种常见功能性VEGF基因多态性（-634G/C和936C/T）与自发性早产之间的可能关系。
方法
从54例早产孕妇和79例至少有两次足月自然分娩的绝经后妇女的全血中提取基因组DNA。通过聚合酶链反应-限制性片段长度多态性（PCR-RFLP）分析DNA样本。
结果
与具有936C/C基因型的个体相比，具有936T/T或936C/T基因型的个体与早产之间存在统计学上的显著关联[P = 0.0009，风险因子2.05，95%置信区间（CI）1.37 - 3.06]。自发性早产与-634基因型之间无显著关联。
结论
VEGF 936C/T多态性与妊娠37周前分娩之间存在关联。
Zotero 插件