Iversen C, Lane M, Forsythe S J
School of Science, The Nottingham Trent University, Clifton Lane, Nottingham, UK.
Lett Appl Microbiol. 2004;38(5):378-82. doi: 10.1111/j.1472-765X.2004.01507.x.
To study the growth, thermotolerance and biofilm formation of the emergent pathogen Enterobacter sakazakii in infant formula milk (IFM).
The temperature range, death kinetics and biofilm formation of E. sakazakii were determined using impedance microbiology and conventional methods. In IFM the organism grew as low as 6 degrees C and optimally at 37-43 degrees C. In faecal coliform tests, 23% of strains (n = 70) produced gas from lauryl sulphate broth (LSB) at 44 degrees C after 48 h incubation. Three strains failed to grow in LSB at any of the temperatures. The D-value of cells suspended in IFM was determined between 54 and 62 degrees C. The resultant z-value was 5.7 degrees C. The organism was able to adhere and grow on latex, polycarbonate, silicon and to a lesser extent stainless steel.
Enterobacter sakazakii was able to grow at refrigeration temperatures and on infant-feeding equipment. The thermotolerance of the organism was similar to other Enterobacteriaceae and should be killed during standard pasteurization treatment.
Enterobacter sakazakii has been associated with infant meningitis through consumption of contaminated IFM. Enterobacter sakazakii is able to grow in IFM during storage at refrigeration temperatures and attach to infant-feeding equipment, which may become reservoirs of infection.
研究新兴病原菌阪崎肠杆菌在婴儿配方奶粉（IFM）中的生长、耐热性及生物膜形成情况。
采用阻抗微生物学和传统方法测定阪崎肠杆菌的温度范围、死亡动力学及生物膜形成情况。在IFM中，该菌在低至6℃时仍能生长，在37 - 43℃时生长最佳。在粪大肠菌群检测中，23%的菌株（n = 70）在44℃下于月桂基硫酸盐肉汤（LSB）中培养48小时后产气。有三株菌在任何温度下均无法在LSB中生长。测定了悬浮于IFM中的细胞在54至62℃之间的D值。所得z值为5.7℃。该菌能够在乳胶、聚碳酸酯、硅上附着并生长，在不锈钢上的附着程度较低。
阪崎肠杆菌能够在冷藏温度下以及婴儿喂养设备上生长。该菌的耐热性与其他肠杆菌科细菌相似，在标准巴氏杀菌处理过程中应可被杀灭。
阪崎肠杆菌通过食用受污染的IFM与婴儿脑膜炎有关。阪崎肠杆菌能够在IFM冷藏储存期间生长，并附着于婴儿喂养设备上，这些设备可能成为感染源。
