Lu Ming, Yang Bei, Cai Ying-yun
Department of Medicine, Zhongshan Hospital, Fudan University, Shanghai 200032, China.
Zhonghua Nei Ke Za Zhi. 2004 Feb;43(2):117-20.
To determine the association between vitamin D binding protein (VDBP) gene and chronic obstructive pulmonary disease (COPD) susceptibility in Han Nationality in China.
This was a case-control study. Blood samples were taken from sixty-nine smokers with COPD (group A) and fifty-two smokers without COPD (Group B). DNA was extracted from the white blood cells of both groups. Genotypes of VDBP were measured with polymerase chain reaction and restriction fragment length polymorphism analysis.
In group A the proportion of allele 1F homozygote was significantly Higher than that in group B (33.3% versus 11.5%, p = 0.005). The odds ratio for 1F-1F was 3.5 (95%confidence interval 1.9294-9.429) for COPD. Phenotype 2-2 showed a decreasing trend in group A, but the difference was not significant due to the small sample size. The frequency of allele 1F in group A was 0.558 compared with 0.404 in group B (p = 0.018). The frequency of allele 2 in group A was 0.196 compared with 0.308 in group B (p = 0.044).
Our result suggests that allele 1F is one of the risk factors for COPD associated with smoking. 1F homozygote may increase the risk of COPD. On the other hand, allele 2 might have a protective effect on pathogenesis of COPD.
确定维生素D结合蛋白（VDBP）基因与中国汉族慢性阻塞性肺疾病（COPD）易感性之间的关联。
这是一项病例对照研究。从69名患有COPD的吸烟者（A组）和52名无COPD的吸烟者（B组）采集血样。从两组的白细胞中提取DNA。采用聚合酶链反应和限制性片段长度多态性分析测定VDBP的基因型。
A组中1F等位基因纯合子的比例显著高于B组（33.3%对11.5%，p = 0.005）。COPD患者中1F - 1F的优势比为3.5（95%置信区间1.9294 - 9.429）。2 - 2表型在A组呈下降趋势，但由于样本量小差异不显著。A组中1F等位基因频率为0.558，B组为0.404（p = 0.018）。A组中2等位基因频率为0.196，B组为0.308（p = 0.044）。
我们的结果表明，1F等位基因是与吸烟相关的COPD危险因素之一。1F纯合子可能增加患COPD的风险。另一方面，2等位基因可能对COPD的发病机制具有保护作用。
