Microbial Exopolysaccharide Research Group, Department of Microbiology, Faculty of Pharmacy, Cartuja Campus, University of Granada, 18071 Granada, Spain.
Mar Drugs. 2010 Jul 30;8(8):2240-51. doi: 10.3390/md8082240.
We have studied the exopolysaccharide produced by the type strain of Salipiger mucosus, a species of halophilic, EPS-producing (exopolysaccharide-producing) bacterium belonging to the Alphaproteobacteria. The strain, isolated on the Mediterranean seaboard, produced a polysaccharide, mainly during its exponential growth phase but also to a lesser extent during the stationary phase. Culture parameters influenced bacterial growth and EPS production. Yield was always directly related to the quantity of biomass in the culture. The polymer is a heteropolysaccharide with a molecular mass of 250 kDa and its components are glucose (19.7%, w/w), mannose (34%, w/w), galactose (32.9%, w/w) and fucose (13.4%, w/w). Fucose and fucose-rich oligosaccharides have applications in the fields of medicine and cosmetics. The chemical or enzymatic hydrolysis of fucose-rich polysaccharides offers a new efficient way to process fucose. The exopolysaccharide in question produces a solution of very low viscosity that shows pseudoplastic behavior and emulsifying activity on several hydrophobic substrates. It also has a high capacity for binding cations and incorporating considerable quantities of sulfates, this latter feature being very unusual in bacterial polysaccharides.
我们研究了 Salipiger mucosus 模式菌株所产生的胞外多糖，该菌是一种嗜盐、产 EPS（胞外多糖）的细菌，属于α变形菌。该菌株从地中海沿岸分离得到，主要在指数生长期产生多糖，但在静止期也有少量产生。培养参数影响细菌的生长和 EPS 的产生。产量始终与培养物中的生物量直接相关。该聚合物是一种异多糖，分子量为 250 kDa，其成分包括葡萄糖（19.7%，w/w）、甘露糖（34%，w/w）、半乳糖（32.9%，w/w）和岩藻糖（13.4%，w/w）。岩藻糖和富含岩藻糖的低聚糖在医学和化妆品领域有应用。富含岩藻糖的多糖的化学或酶水解为加工岩藻糖提供了一种新的有效方法。所研究的胞外多糖产生一种低粘度的溶液，表现出假塑性行为，并对几种疏水性底物具有乳化活性。它还具有结合阳离子和掺入大量硫酸盐的高能力，这一特征在细菌多糖中非常罕见。
