Hernandez-Andrade E, Jansson T, Ley D, Bellander M, Persson M, Lingman G, Marsál K
Department of Obstetrics and Gynecology, University of Lund, Lund, Sweden.
Ultrasound Obstet Gynecol. 2004 Apr;23(4):363-8. doi: 10.1002/uog.1002.
To compare fractional moving blood volume (FMBV) estimation using power Doppler ultrasound (PDU) with blood flow estimation using radioactive microspheres (RMS) for evaluation of fetal organ blood perfusion.
Blood flow was measured in the adrenal gland of nine exteriorized fetal lambs. Five fetal lambs underwent total umbilical cord occlusion in order to induce changes in the adrenal blood flow (asphyxia group). Four lambs were used as sham controls (control group). Three RMS injections, with coincident PDU recordings of the adrenal gland, were performed in each lamb. In the asphyxia group, measurements were taken before the cord occlusion, 5 min later and when the mean blood pressure decreased below 25 mmHg. In the control group, the measurements were done with an interval of 5 min. FMBV normalized for attenuation of PDU signals, and mean pixel intensity (MPI) were estimated offline. After completion of the study, adrenal blood perfusion was calculated according to the reference sample microsphere technique, using the isotope activity and expressed in mL/min/100 g. The correlation between RMS and FMBV and MPI, respectively, was analyzed individually for each lamb.
In the asphyxia group, all lambs showed a marked reduction in the adrenal blood perfusion towards the third RMS injection. In the control group, the adrenal perfusion showed small variations throughout the experiment. In the total material, there was a higher correlation between FMBV and RMS (median, r = 0.90; range, 0.43-0.99) than between MPI and RMS (median, r = 0.55; range, -0.53 to 0.99).
The FMBV method of quantifying PDU signals correlates highly with blood flow perfusion estimation using RMS in the fetal lamb adrenal gland.
比较使用功率多普勒超声（PDU）估计的分数移动血容量（FMBV）与使用放射性微球（RMS）进行的血流估计，以评估胎儿器官的血液灌注。
对9只体外胎儿羔羊的肾上腺进行血流测量。5只胎儿羔羊进行完全脐带结扎以诱导肾上腺血流变化（窒息组）。4只羔羊作为假手术对照组（对照组）。每只羔羊进行3次RMS注射，并同时记录肾上腺的PDU图像。在窒息组中，在脐带结扎前、结扎后5分钟以及平均血压降至25 mmHg以下时进行测量。在对照组中，测量间隔为5分钟。离线估计经PDU信号衰减校正的FMBV和平均像素强度（MPI）。研究完成后，根据参考样品微球技术，使用同位素活性计算肾上腺血流灌注，并以mL/min/100 g表示。分别分析每只羔羊RMS与FMBV和MPI之间的相关性。
在窒息组中，所有羔羊在第三次RMS注射时肾上腺血流灌注均显著降低。在对照组中，整个实验过程中肾上腺灌注变化较小。在全部样本中，FMBV与RMS之间的相关性（中位数，r = 0.90；范围，0.43 - 0.99）高于MPI与RMS之间的相关性（中位数，r = 0.55；范围，-0.53至0.99）。
在胎儿羔羊肾上腺中，量化PDU信号的FMBV方法与使用RMS进行的血流灌注估计高度相关。
