Pasqualotto Eleonora Bedin, Lara Luana Venturin, Salvador Mirian, Sobreiro Bernardo Passos, Borges Edson, Pasqualotto Fábio Firmbach
University of Caxias do Sul, Brazil.
Hum Fertil (Camb). 2009;12(3):166-71. doi: 10.1080/14647270903207941.
The follicular fluid environment surrounding the oocytes may play a critical role in fertilization and subsequent embryo development. The goal of our study was to evaluate the oxidative stress markers in the semen, blood serum, and follicular fluid of couples undergoing Intracytoplasmic Sperm Injection (ICSI). Two hundred and eight infertile couples underwent ICSI and the levels of superoxide dismutase (SOD) and catalase and lipid peroxidation (LPO) were evaluated. Semen Catalase was highly correlated with fertilization and cleavage rates, but not with pregnancy rates. Fertilization and cleavage rates were correlated with the levels of SOD and Catalase in the follicular fluid. After adjusting for age, a negative correlation was detected between LPO levels in follicular fluid and pregnancy rates. Follicular fluid LPO levels may be a marker as a metabolic activity within the follicle need for establishing a pregnancy.
卵母细胞周围的卵泡液环境可能在受精及后续胚胎发育过程中发挥关键作用。我们研究的目的是评估接受卵胞浆内单精子注射（ICSI）的夫妇精液、血清和卵泡液中的氧化应激标志物。208对不育夫妇接受了ICSI治疗，并评估了超氧化物歧化酶（SOD）、过氧化氢酶水平及脂质过氧化（LPO）情况。精液过氧化氢酶与受精率和卵裂率高度相关，但与妊娠率无关。受精率和卵裂率与卵泡液中SOD和过氧化氢酶水平相关。校正年龄后，卵泡液中LPO水平与妊娠率呈负相关。卵泡液LPO水平可能是卵泡内建立妊娠所需代谢活动的一个标志物。
