卵泡液活性氧对体外受精结局的影响。

The effect of follicular fluid reactive oxygen species on the outcome of in vitro fertilization.

作者信息

Attaran M, Pasqualotto E, Falcone T, Goldberg J M, Miller K F, Agarwal A, Sharma R K

机构信息

Center for Advanced Research in Human Reproduction and Infertility, Department of Gynecology and Obstetrics, The Cleveland Clinic Foundation, Ohio 44195, USA.

出版信息

Int J Fertil Womens Med. 2000 Sep-Oct;45(5):314-20.

PMID:11092702

Abstract

OBJECTIVE

To examine the presence of reactive oxygen species (ROS) in the follicular fluid of women undergoing in vitro fertilization (IVF) and identify its role in pregnancy outcome.

STUDY DESIGN

In this prospective study, ROS and total antioxidant capacity (TAC) levels were measured by the chemiluminescence method in the follicular fluid of 53 women. Age, number of oocytes recovered, percentage of oocytes fertilized, ROS and TAC levels were compared in women who did and did not become pregnant.

RESULTS

Patients who become pregnant had significantly higher log-transformed ROS levels (1.01 +/- 0.14, P = 0.031 than those who did not (0.69 +/- 0.08). Women with endometriosis or male factor infertility who became pregnant had significantly higher ROS levels (1.44 +/- 0.23 and 1.31 +/- 0.19) than those who did not (0.60 +/- 0.17 and 0.67 +/- 0.16; P < .006 and P < .01).

CONCLUSION

Follicular fluid ROS, at low concentrations, may be a potential marker for predicting success in IVF patients.

摘要

目的

检测接受体外受精（IVF）的女性卵泡液中活性氧（ROS）的存在情况，并确定其在妊娠结局中的作用。

研究设计

在这项前瞻性研究中，采用化学发光法测定了53名女性卵泡液中的ROS和总抗氧化能力（TAC）水平。比较了妊娠和未妊娠女性的年龄、回收的卵母细胞数量、受精的卵母细胞百分比、ROS和TAC水平。

结果

妊娠患者经对数转换后的ROS水平（1.01±0.14，P = 0.031）显著高于未妊娠患者（0.69±0.08）。患有子宫内膜异位症或男性因素不孕症且妊娠的女性ROS水平（1.44±0.23和1.31±0.19）显著高于未妊娠女性（0.60±0.17和0.67±0.16；P <.006和P <.01）。