Duverneuil Charlotte, de la Grandmaison Geoffroy Lorin, de Mazancourt Philippe, Alvarez Jean-Claude
Laboratoire de Pharmacologie-Toxicologie, Centre Hospitalier Universitaire Raymond Poincaré, 104 Boulevard R. Poincaré, 92380 Garches, France.
Forensic Sci Int. 2004 Apr 20;141(1):17-21. doi: 10.1016/j.forsciint.2003.12.010.
An original liquid chromatography method with photodiode-array detection (DAD) is presented for the determination of strychnine in blood. This sensitive method allows the use of only 0.1 ml of sample. The strychnine was isolated from blood using a liquid-liquid extraction procedure and chloroquine as an internal standard. The limits of detection (LOD) and quantification were 0.06 and 0.5 mg/l, respectively. The recovery was 94% and the coefficients of variation (CV) ranged from 5.9 to 10.8%. A fatal case of strychnine poisoning is presented, with a lethal blood concentration of 25 mg/l.
本文介绍了一种采用光电二极管阵列检测（DAD）的液相色谱原始方法，用于测定血液中的士的宁。这种灵敏的方法仅需0.1毫升样品。通过液液萃取程序并以氯喹作为内标从血液中分离出士的宁。检测限（LOD）和定量限分别为0.06和0.5毫克/升。回收率为94%，变异系数（CV）在5.9%至10.8%之间。本文还介绍了一例士的宁中毒致死病例，其致死血液浓度为25毫克/升。
