Ferranti Pasquale, Traisci Maria Vittoria, Picariello Gianluca, Nasi Antonella, Boschi Velia, Siervo Mario, Falconi Claudio, Chianese Lina, Addeo Francesco
Dipartimento di Scienza degli Alimenti, Università degli Studi di Napoli Federico II, Portici, Italy.
J Dairy Res. 2004 Feb;71(1):74-87. doi: 10.1017/s0022029903006599.
The protein and peptide fraction of human milk samples collected from mothers of pre- and full-term infants in the first week after parturition was analysed by use of liquid chromatography-mass spectrometry and tandem mass spectrometry. By characterising the peptide sequence, we defined the pathway of casein hydrolysis which leads to the formation of small peptides through intermediate oligopeptides. It was found that the action of a plasmin-like enzyme acting on specific lysine residues is the primary step in casein degradation. This is followed by endopeptidases and/or exopeptidases mediated cleavage of the oligopeptides which, in turn, produces a multiplicity of short peptides differing by one or more amino acid residues. In this process, a series of potentially bioactive peptides (opioid, phosphopeptides) and their precursors are produced.
采用液相色谱 - 质谱联用和串联质谱法对分娩后第一周从早产和足月产婴儿母亲采集的母乳样本中的蛋白质和肽组分进行了分析。通过对肽序列进行表征，我们确定了酪蛋白水解途径，该途径通过中间寡肽导致小肽的形成。结果发现，一种作用于特定赖氨酸残基的类纤溶酶的作用是酪蛋白降解的第一步。随后是内肽酶和/或外肽酶介导的寡肽裂解，进而产生多种相差一个或多个氨基酸残基的短肽。在此过程中，产生了一系列潜在的生物活性肽（阿片样肽、磷酸肽）及其前体。
