Salvat A, Antonacci L, Fortunato R H, Suarez E Y, Godoy H M
Instituto de Patobiología, Centro Nacional de Investigaciones Agropecuarias, INTA-Castelar, Pcia. de Buenos Aires, Argentina.
Phytomedicine. 2004 Feb;11(2-3):230-4. doi: 10.1078/0944-7113-00327.
Thirty-nine native plant species were collected from the provinces of Chaco and Formosa, in northern Argentina, and were screened for antimicrobial activity. The plants were dried and extracted thoroughly with methanol. The dry extracts, dissolved in dimethylsulfoxide, were tested for inhibition of microbial growth via microplate assay with an oxidation-reduction dye. The test organisms were: Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus aureus and Enterococcus faecium. Inhibition of respiratory activities in some of these microbial species was produced by the extracts of Astronium balansae, Geoffroea decorticans, Peltophorum dubium, Geoffroea spinosa, Lantana balansae, Prosopis kuntzei, Prosopis ruscifolia and Bulnesia sarmientoi, with minimal inhibitory concentrations (MIC) ranging from 0.08 to 0.5 mg dry matter/ml. Further in vitro experiments measuring the growth of S. aureus in liquid culture confirmed that all of the above extracts at 2 x MIC were able to inhibit bacterial growth effectively, and that some of them (A. balansae, G. decorticans, P. dubium, G. spinosa, P. kuntzei and B. sarmientoi) were able to reduce the initial number of viable counts by at least one order of magnitude in 10 hours, indicating that these extracts should be investigated further for the possible presence of bactericidal components.
从阿根廷北部的查科省和福尔摩沙省采集了39种本地植物物种，并对其进行了抗菌活性筛选。将这些植物干燥后用甲醇彻底提取。将干燥提取物溶解在二甲基亚砜中，通过使用氧化还原染料的微孔板试验测试其对微生物生长的抑制作用。测试的微生物有：大肠杆菌、鼠伤寒沙门氏菌、铜绿假单胞菌、肺炎克雷伯菌、金黄色葡萄球菌和粪肠球菌。巴拉那阿斯托木、去皮吉奥弗罗木、钝叶盾柱木、具刺吉奥弗罗木、巴拉那马缨丹、昆茨牧豆树、鲁斯西牧豆树和萨尔缅托布尔内西亚的提取物对其中一些微生物物种的呼吸活动产生了抑制作用，最低抑菌浓度（MIC）范围为0.08至0.5毫克干物质/毫升。进一步测量金黄色葡萄球菌在液体培养物中生长的体外实验证实，所有上述提取物在2倍MIC浓度下均能有效抑制细菌生长，其中一些提取物（巴拉那阿斯托木、去皮吉奥弗罗木、钝叶盾柱木、具刺吉奥弗罗木、昆茨牧豆树和萨尔缅托布尔内西亚）能够在10小时内将活菌数初始数量至少降低一个数量级，这表明应对这些提取物进一步研究，以确定是否可能存在杀菌成分。
