Enderlein Jörg, Gregor Ingo, Patra Digambara, Fitter Jörg
Institute for Biological Information Processing 1 & 2, Forschungszentrum Jülich, D-52425 Jülich, Germany.
Curr Pharm Biotechnol. 2004 Apr;5(2):155-61. doi: 10.2174/1389201043377020.
Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.
荧光相关光谱法（FCS）是研究溶液中低浓度分析物分子的一项重要技术。FCS能够测定的核心分子特性是分析物分子的平移扩散系数，该系数可用于研究分子结合与反应，或大分子的构象变化等。本文讨论了几种可能影响FCS测量结果从而使导出的扩散系数值产生偏差的光学和光物理效应。
