Nelson-Filho Paulo, Isper Alessandra Rigo, Assed Sada, Faria Gisele, Ito Izabel Yoko
Department of Pediatric Dentistry, School of Dentistry of Ribeirão Preto, University of São Paulo, Brazil.
Pediatr Dent. 2004 Jan-Feb;26(1):11-6.
The objective of this study was to evaluate by culture and scanning electron microscopy (SEM) the contamination of toothbrushes of 30 children (5-7 years old) by mutans streptococci (MS) when dentifrices with or without triclosan are used.
The clinical procedures were divided into 3 phases at 1-week intervals. In phase 1 (group I), the children brushed their teeth without dentifrice for 4 minutes; phase 2 (group II) brushed with fluoridated dentifrice (Tandy); phase 3 (group III) brushed with dentifrice containing triclosan (Colgate Total). The toothbrushes were then submitted to microbiological processing for the counting of colony-forming units (CFUs) of MS adhered to the bristles. Four toothbrushes from each group were analyzed by SEM.
MS were present on 93% of group I toothbrush bristles and on 77% of group II toothbrush bristles. Only 40% of group III toothbrushes were contaminated with MS. When there was a positive microbiological culture, the formation of cariogenic bacterial biofilm adhered to the bristles of all groups was identified by SEM.
Toothbrush bristles were contaminated by MS after only one use. A dentifrice containing triclosan significantly reduced bacterial contamination of these toothbrushes.
本研究的目的是通过培养和扫描电子显微镜（SEM）评估30名5至7岁儿童在使用含或不含三氯生的牙膏时，变形链球菌（MS）对牙刷的污染情况。
临床程序分为3个阶段，间隔1周。在第1阶段（I组），儿童不使用牙膏刷牙4分钟；第2阶段（II组）使用含氟牙膏（坦迪）刷牙；第3阶段（III组）使用含三氯生的牙膏（高露洁全效）刷牙。然后将牙刷进行微生物处理，以计数附着在刷毛上的MS的菌落形成单位（CFU）。对每组的4把牙刷进行SEM分析。
I组93%的牙刷刷毛上存在MS，II组77%的牙刷刷毛上存在MS。只有40%的III组牙刷被MS污染。当微生物培养呈阳性时，通过SEM鉴定出所有组的刷毛上均有产酸细菌生物膜形成。
牙刷仅使用一次后刷毛就被MS污染。含三氯生的牙膏显著减少了这些牙刷的细菌污染。
