Sikorski R Steven, Wang Lin, Markham Kelli A, Rajagopalan P T Ravi, Benkovic Stephen J, Kohen Amnon
Department of Chemistry, University of Iowa, Iowa City, Iowa 52242, USA.
J Am Chem Soc. 2004 Apr 21;126(15):4778-9. doi: 10.1021/ja031683w.
H-transfer was studied in the complex kinetic cascade of dihydrofolate reductase. Intrinsic kinetic isotope effects, their temperature dependence, and other temperature-dependent parameters indicated H-tunneling, but no 1 degrees to 2 degrees coupled motion. The data also suggested environmentally coupled tunneling and commitment to catalysis on pre-steady-state isotope effects.
在二氢叶酸还原酶的复杂动力学级联反应中研究了氢转移。本征动力学同位素效应、它们对温度的依赖性以及其他温度依赖性参数表明存在氢隧穿，但不存在1°到2°的耦合运动。数据还表明存在环境耦合隧穿以及对稳态前同位素效应催化作用的投入。
