Bewley T A, Li C H
Biochemistry. 1978 Aug 8;17(16):3315-20. doi: 10.1021/bi00609a022.
The dissociation of tetra-S-carbamidomethylated, plasmin-modified human somatotropin at 25 +/- 2 degrees C has been found to be a slow, exergonic equilibrium reaction; an equilibrium constant of 1.77 mol/L and a standard free energy of dissociation of -340 cal/mol have been calculated. The major products of the dissociation have been shown to consist of a monomeric form of the 51-residue carboxyl-terminal fragment and a trimeric form of the 134-residue amino-terminal fragment. The carboxyl-terminal fragment appears to be a random coil, while the amino-terminal fragment retains a considerable degree of secondary and tertiary structures.
已发现，在25±2℃下，经纤溶酶修饰的四-S-氨甲酰甲基化人生长激素的解离是一个缓慢的、放能的平衡反应；已计算出平衡常数为1.77 mol/L，标准解离自由能为-340 cal/mol。已表明解离的主要产物由51个残基的羧基末端片段的单体形式和134个残基的氨基末端片段的三聚体形式组成。羧基末端片段似乎是无规卷曲，而氨基末端片段保留了相当程度的二级和三级结构。
