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1
Human somatotropin: biological characterization of the recombinant molecule.人生长激素：重组分子的生物学特性
Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5. doi: 10.1073/pnas.73.10.3463.
2
Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.
3
Human pituitary growth hormone: restoration of full biological activity by noncovalent interaction of two fragments of the hormone.人生长激素：通过激素两个片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1976 May;73(5):1476-9. doi: 10.1073/pnas.73.5.1476.
4
Human pituitary growth hormone. 44. Effects of plasmin-modified hormone and its fragments on ornithine decarboxylase activity and lipolysis.人生长激素。44. 纤溶酶修饰的激素及其片段对鸟氨酸脱羧酶活性和脂肪分解的影响。
Proc Soc Exp Biol Med. 1976 Feb;151(2):285-7. doi: 10.3181/00379727-151-39193.
5
Tissue-specific stimulation of ornithine decarboxylase activity by pituitary factors immunologically related to growth hormone.垂体中与生长激素免疫相关的因子对鸟氨酸脱羧酶活性的组织特异性刺激作用。
Endocrinology. 1977 Feb;100(2):459-67. doi: 10.1210/endo-100-2-459.
6
Two contiguous thrombin fragments of human somatotropin form a functionally active recombinant, but the two homologous fragments from sheep hormone do not.人生长激素的两个相邻凝血酶片段形成了一种功能活跃的重组体，但来自绵羊激素的两个同源片段却不能。
Biochemistry. 1981 Dec 8;20(25):7251-8. doi: 10.1021/bi00528a031.
7
Human pituitary growth hormone: a biologically active hendekakaihekaton peptide fragment corresponding to amino-acid residues 15-125 in the hormone molecule.人垂体生长激素：一种具有生物活性的一百二十一肽片段，对应于激素分子中的氨基酸残基15 - 125。
Proc Natl Acad Sci U S A. 1975 Oct;72(10):3878-82. doi: 10.1073/pnas.72.10.3878.
8
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
9
Isolation and characterization of fragments of reduced and S-carbamidomethylated human growth hormone produced by plasmin digestion. II. Biological and immunological activities.纤溶酶消化产生的还原型和S-氨甲酰甲基化人生长激素片段的分离与特性鉴定。II. 生物学和免疫学活性。
Endocrinology. 1978 May;102(5):1377-86. doi: 10.1210/endo-102-5-1377.
10
Chemical messengers of the adenohypophysis from somatotropin to lipotropin. Herbert M. Evans Memorial Lecture, 1976.从生长激素到促脂解素的腺垂体化学信使。1976年赫伯特·M·埃文斯纪念讲座
Perspect Biol Med. 1978 Spring;21(3):447-65. doi: 10.1353/pbm.1978.0034.

引用本文的文献

1
Human growth hormone: 1974-1981.人生长激素：1974 - 1981年。
Mol Cell Biochem. 1982 Jul 7;46(1):31-41. doi: 10.1007/BF00215579.
2
Semisynthesis of human somatotropin analogs.人生长激素类似物的半合成
Proc Natl Acad Sci U S A. 1979 Dec;76(12):6124-7. doi: 10.1073/pnas.76.12.6124.
3
Noncovalent interaction of the NH2-terminal fragment of human somatotropin with the COOH-terminal fragment of human choriomammotropin to generate growth-promoting activity.人生长激素的氨基末端片段与人类绒毛膜促乳腺生长激素的羧基末端片段发生非共价相互作用，以产生促生长活性。
Proc Natl Acad Sci U S A. 1978 Apr;75(4):1700-2. doi: 10.1073/pnas.75.4.1700.
4
Human somatotropin: restoration of full biological activity by noncovalent interaction of a natural and a synthetic fragment of the hormone.人生长激素：通过激素的天然片段与合成片段的非共价相互作用恢复完全生物活性。
Proc Natl Acad Sci U S A. 1977 Mar;74(3):1016-9. doi: 10.1073/pnas.74.3.1016.
5
Two fragments from fibrinolysin digests of ovine prolactin: characterization and recombination to generate full immunoreactivity.绵羊催乳素纤维蛋白溶酶消化产生的两个片段：特性鉴定及重组以产生完全免疫反应性
Proc Natl Acad Sci U S A. 1978 May;75(5):2155-9. doi: 10.1073/pnas.75.5.2155.

本文引用的文献

1
THE PREPARATION OF I-131-LABELLED HUMAN GROWTH HORMONE OF HIGH SPECIFIC RADIOACTIVITY.高比放射性碘-131标记人生长激素的制备
Biochem J. 1963 Oct;89(1):114-23. doi: 10.1042/bj0890114.
2
Human pituitary growth hormone. VI. Modified procedure of isolation and NH2-terminal amino acid sequence.人垂体生长激素。VI. 改进的分离方法及氨基末端氨基酸序列
Arch Biochem Biophys. 1962 Sep;Suppl 1:327-32.
3
Plasma growth hormone concentration in the rat determined by radioimmunoassay: influence of sex, pregnancy, lactation, anesthesia, hypophysectomy and extrasellar pituitary transplants.用放射免疫分析法测定大鼠血浆生长激素浓度：性别、妊娠、哺乳、麻醉、垂体切除及垂体鞍外移植的影响。
Endocrinology. 1966 Aug;79(2):275-80. doi: 10.1210/endo-79-2-275.
4
On the purification of L-ornithine decarboxylase from rat prostate and effects of thiol compounds on the enzyme.大鼠前列腺L-鸟氨酸脱羧酶的纯化及硫醇化合物对该酶的影响
J Biol Chem. 1971 Mar 25;246(6):1725-32.
5
Human pituitary growth hormone: immunochemical investigations of biologically active fragments.人垂体生长激素：生物活性片段的免疫化学研究
Arch Biochem Biophys. 1974 Oct;164(2):571-4. doi: 10.1016/0003-9861(74)90068-x.
6
Human pituitary growth hormone: isolation and properties of two biologically active fragments from plasmin digests.人垂体生长激素：纤溶酶消化产物中两个生物活性片段的分离与特性
Proc Natl Acad Sci U S A. 1974 Apr;71(4):1197-201. doi: 10.1073/pnas.71.4.1197.
7
Metabolic effects of plasmin digests of human growth hormone in the rat and man.人生长激素纤溶酶消化产物对大鼠和人的代谢影响。
J Clin Invest. 1973 Nov;52(11):2941-51. doi: 10.1172/JCI107491.
8
Isohormones of human growth hormone. II. Plasmin-catalyzed transformation and increase in prolactin biological activity.人生长激素的同功激素。II. 纤溶酶催化的转化及催乳素生物活性的增加。
Endocrinology. 1973 Oct;93(4):858-65. doi: 10.1210/endo-93-4-858.
9
Human pituitary growth hormone. 44. Effects of plasmin-modified hormone and its fragments on ornithine decarboxylase activity and lipolysis.人生长激素。44. 纤溶酶修饰的激素及其片段对鸟氨酸脱羧酶活性和脂肪分解的影响。
Proc Soc Exp Biol Med. 1976 Feb;151(2):285-7. doi: 10.3181/00379727-151-39193.
10
Isolation and biological characterization of fragments of human growth hormone produced by digestion with plasmin.用纤溶酶消化产生的人生长激素片段的分离与生物学特性研究
Endocrinology. 1975 Mar;96(3):625-36. doi: 10.1210/endo-96-3-625.

人生长激素：重组分子的生物学特性

Human somatotropin: biological characterization of the recombinant molecule.

作者信息

Li C H, Hayashida T, Doneen B A, Rao A J

出版信息

Proc Natl Acad Sci U S A. 1976 Oct;73(10):3463-5. doi: 10.1073/pnas.73.10.3463.

DOI:10.1073/pnas.73.10.3463
PMID:62361
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC431135/
Abstract

The recombinant hormone obtained by non-covalent interaction of the NH2-terminal 134 amino acid fragment with the COOH-terminal 51 amino acid fragment of the reduced-carbamidomethylated human somatotropin molecule is found to exhibit nearly full biological activity of the native hormone, as evidenced by the stimulation of hepatic ornithine decarboxylase (L-ornithine carboxy-lyase, EC 4.1.1.17) in vivo and protein synthesis in mouse mammary gland in vitro. Radioimmunoassay data indicate that the recombinant behaves immunochemically in a manner almost identical to that of the native hormone.

摘要

通过还原氨甲酰甲基化人生长激素分子的NH2末端134个氨基酸片段与COOH末端51个氨基酸片段的非共价相互作用获得的重组激素，被发现具有近乎天然激素的全部生物活性，这在体内对肝鸟氨酸脱羧酶（L-鸟氨酸羧基裂解酶，EC 4.1.1.17）的刺激以及体外对小鼠乳腺蛋白质合成的刺激中得到了证明。放射免疫分析数据表明，该重组体在免疫化学行为上几乎与天然激素相同。