Mogelsvang Soren, Marsh Brad J, Ladinsky Mark S, Howell Kathryn E
University of Colorado School of Medicine, Department of Cell and Developmental Biology, University of Colorado Health Sciences Center, Denver, CO 80262, USA.
Traffic. 2004 May;5(5):338-45. doi: 10.1111/j.1398-9219.2004.00186.x.
3D electron tomography studies of the structure of the mammalian Golgi complex have led to four functional predictions (1). The sorting and exit site from the Golgi comprises two or three distinct trans-cisternae (2). The docking of vesicular-tubular clusters at the cis-face and the fragmentation of trans-cisternae are coordinated (3). The mechanisms of transport through, and exit from, the Golgi vary with physiological state, and in different cells and tissues (4). Specialized trans-ER functions in the delivery of ceramide to sphingomyelin synthase in the trans-Golgi membrane, for the regulated sorting via sphingolipid-cholesterol-rich domains. These structure-based predictions can now be tested using a variety of powerful cell and molecular tools.
对哺乳动物高尔基体复合体结构的三维电子断层扫描研究得出了四项功能预测（1）。高尔基体的分选和出口位点由两到三个不同的反式扁平囊组成（2）。囊泡管状簇在顺面的对接与反式扁平囊的碎片化是协调进行的（3）。通过高尔基体运输及从高尔基体出口的机制随生理状态以及不同细胞和组织而变化（4）。内质网在反式高尔基体膜中将神经酰胺递送至鞘磷脂合酶方面具有特殊的反式功能，用于通过富含鞘脂 - 胆固醇的结构域进行调节性分选。现在可以使用各种强大的细胞和分子工具来检验这些基于结构的预测。
