Young Lei, Dong Qihan
Department of Medicine, University of Sydney, Australia.
Nucleic Acids Res. 2004 Apr 15;32(7):e59. doi: 10.1093/nar/gnh058.
In the post-genomic era, the ability to synthesize any arbitrary DNA sequence is increasingly in demand. A bottleneck in current gene synthesis technologies is the associated cost, due primarily to the high cost of oligonucleotides synthesis and post-synthesis sequencing. In the present paper, an improved method for low-cost gene synthesis that combines dual asymmetrical PCR and overlap extension PCR is presented, which enables any DNA sequence to be synthesized error free. Additionally, the method is easily amenable to automation.
在后基因组时代，合成任意DNA序列的能力需求日益增加。当前基因合成技术的一个瓶颈是相关成本，这主要归因于寡核苷酸合成和合成后测序的高成本。本文提出了一种结合双重不对称PCR和重叠延伸PCR的低成本基因合成改进方法，该方法能够无错误地合成任何DNA序列。此外，该方法易于实现自动化。
