Kong Z M, Zang Y, Wu Q L
National Key Laboratory of Pollution Control and Resource Reuse of China, Department of Environmental Science and Engineering, Nanjing University, Nanjing 210093, People's Republic of China.
Environ Pollut. 1998;99(2):279-83. doi: 10.1016/s0269-7491(97)00111-5.
Mutagenicity of surface water in Lake Taihu was monitored by genotoxicological methods. The goal is to determine the situation concerning the contamination by mutagens, their distribution in the lake, and the potential effect on public health. In this study, the micronucleus test of Vicia faba root tip, a standard monitoring technique now in China, was carried out to make a preliminary screening of environmental mutagens in 39 representative sites of the lake. Highly significant differences in MCN permillage (average number of micronuclei per 1000 cells) and PI (pollution index) values were found among these sites. From the results, a rough distribution of the contamination could be obtained: mainly concentrated in the north-west area of the lake from several tributaries flowing into the lake. In order to provide further evidence, organic extracts from four entrances and one outlet of the five major tributaries were examined by the micronucleus test of human peripheral lymphocytes. The results were in accord with those of the micronucleus tests using Vicia faba. The tests also revealed that the intensity and mechanism of the pollutants were diverse in the different sources.
采用遗传毒理学方法监测太湖地表水的致突变性。目的是确定诱变剂污染状况、其在湖泊中的分布以及对公众健康的潜在影响。本研究采用中国现行标准监测技术蚕豆根尖微核试验，对太湖39个代表性点位的环境诱变剂进行初步筛选。这些点位的微核率（每千个细胞中微核的平均数）和PI（污染指数）值存在极显著差异。根据结果，可得出污染的大致分布情况：主要集中在湖泊西北部几条流入湖泊的支流区域。为提供进一步证据，对五大支流的四个入口和一个出口的有机提取物进行了人外周血淋巴细胞微核试验检测。结果与蚕豆微核试验结果一致。试验还表明，不同来源污染物的强度和作用机制各不相同。
