来自巴西亚马逊地区的达林按蚊多线染色体上的物理基因Hsp70图谱。

The physical gene Hsp70 map on polytene chromosomes of Anopheles darlingi from the Brazilian Amazon.

作者信息

Rafael Míriam Silva, Tadei Wanderli Pedro, Hunter Fiona F

机构信息

Coordenação de Pesquisas em Ciências da Saúde, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Bairro Petrópolis, 69083-000, Manaus, AM, Brasil.

出版信息

Genetica. 2004 May;121(1):89-94. doi: 10.1023/b:gene.0000019959.45267.d7.

DOI:10.1023/b:gene.0000019959.45267.d7

PMID:15098741

Abstract

In situ hybridization was used to determine the physical location of the Hsp70 genes in salivary polytene chromosomes of Anopheles darlingi from Manaus and Macapá, Brazil, and to assess the usefulness of the Hsp70 locus as a genetic marker in A. darlingi populations. In both populations, the double markings corresponding to the Hsp70-12A and Hsp70-14A genes were located on the right arm of chromosome 2. The Hsp70 locus was considered to be an excellent marker for studying chromosomal evolution and relationships among A. darlingi populations.

摘要

原位杂交技术用于确定来自巴西玛瑙斯和马卡帕的达林按蚊唾液多线染色体中Hsp70基因的物理位置，并评估Hsp70基因座作为达林按蚊种群遗传标记的效用。在这两个种群中，对应于Hsp70 - 12A和Hsp70 - 14A基因的双重标记位于2号染色体的右臂上。Hsp70基因座被认为是研究达林按蚊种群染色体进化及相互关系的优良标记。