（）基因组支架的物理图谱

Physical Mapping of the () Genomic Scaffolds.

作者信息

Rafael Míriam Silva, Bridi Leticia Cegatti, Sharakhov Igor V, Marinotti Osvaldo, Sharakhova Maria V, Timoshevskiy Vladimir, Guimarães-Marques Giselle Moura, Santos Valéria Silva, da Silva Carlos Gustavo Nunes, Astolfi-Filho Spartaco, Tadei Wanderli Pedro

机构信息

Coordenação de Sociedade Ambiente e Saúde, Laboratório de Vetores de Malária e Dengue, Instituto Nacional de Pesquisas da Amazônia, Av. André Araújo, 2936, Manaus, AM 69060-001, Brazil.

Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiv, Instituto Nacional de Pesquisas da Amazônia, Manaus, AM 69060-001, Brazil.

出版信息

Insects. 2021 Feb 15;12(2):164. doi: 10.3390/insects12020164.

DOI:10.3390/insects12020164

PMID:33671870

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7918962/

Abstract

The genome assembly of consists of 2221 scaffolds (N50 = 115,072 bp) and has a size spanning 136.94 Mbp. This assembly represents one of the smallest genomes among species. genomic DNA fragments of ~37 Kb were cloned, end-sequenced, and used as probes for fluorescence in situ hybridization (FISH) with salivary gland polytene chromosomes. In total, we mapped nine DNA probes to scaffolds and autosomal arms. Comparative analysis of the scaffolds with homologous sequences of the and genomes identified chromosomal rearrangements among these species. Our results confirmed that physical mapping is a useful tool for anchoring genome assemblies to mosquito chromosomes.

摘要

[物种名称]的基因组组装由2221个支架序列组成（N50 = 115,072碱基对），大小跨越136.94兆碱基对。该组装代表了[物种名称]中最小的基因组之一。约37千碱基对的[物种名称]基因组DNA片段被克隆、末端测序，并用作与唾液腺多线染色体进行荧光原位杂交（FISH）的探针。我们总共将九个DNA探针定位到支架序列和常染色体臂上。对[物种名称]的支架序列与[其他两个物种名称]基因组的同源序列进行比较分析，确定了这些物种之间的染色体重排。我们的结果证实，物理图谱是将基因组组装锚定到蚊子染色体上的有用工具。