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Transduction of a cosmid with the R4 phage cos sequence by heterogeneous actinophages, SPA10 and SPA38.

作者信息

Morino T, Takahashi H

机构信息

Research and Development Division, Nippon Kayaku Co., Ltd., Tokyo, Japan.

出版信息

Biosci Biotechnol Biochem. 1996 Dec;60(12):2076-7. doi: 10.1271/bbb.60.2076.

DOI:10.1271/bbb.60.2076
PMID:8988643
Abstract

A cosmid, pR4C1, composed of the actinophage R4 cos sequence and Streptomyces plasmid pIJ365, was encapsidated in R4 phage particles in vivo. [T. Morino et al., Mol. Gen. Genet., 198, 228-233 (1985)] In this report a cosmid derivative, pR4C4, is shown to be also encapsidated by heterogeneous actinophages, SPA10 and SPA38, and transferred to Streptomyces lividans. Use of this transduction and conservation of the DNA packaging mechanism are discussed.

摘要

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