Maeda Sumio, Sawamura Akiko, Matsuda Akiko
Food Safety Laboratory, Faculty of Human Life and Environment, Nara Women's University, Kitauoyanishi-machi, 630-8506, Japan.
FEMS Microbiol Lett. 2004 Jul 1;236(1):61-4. doi: 10.1016/j.femsle.2004.05.023.
We report that colonial Escherichia coli cells on various solid media can develop modest genetic competence. Using an on-filter culture system, we found that E. coli colonies on CaCl2-containing agar were transformed in the presence of plasmid DNA. Interestingly, transformation also occurred on LB-agar, various moist foods and even on H2O-agar. These results suggest that some populations of colonial E. coli in various environments could become transformable regardless of the surrounding Ca2+ concentration.
我们报告称，在各种固体培养基上的菌落状大肠杆菌细胞可发展出适度的遗传感受态。使用滤膜培养系统，我们发现含氯化钙的琼脂上的大肠杆菌菌落在存在质粒DNA的情况下会发生转化。有趣的是，在LB琼脂、各种潮湿食物甚至水琼脂上也会发生转化。这些结果表明，在各种环境中，一些菌落状大肠杆菌群体无论周围钙离子浓度如何都可能变得具有转化能力。
