Xu Ting, Logsdon Naomi J, Walter Mark R
Center for Biophysical Sciences and Engineering, University of Alabama at Birmingham, 35294, USA.
Acta Crystallogr D Biol Crystallogr. 2004 Jul;60(Pt 7):1295-8. doi: 10.1107/S0907444904010492. Epub 2004 Jun 22.
Interleukin-22 is a potent mediator of cellular inflammatory responses. Crystals of interleukin-22 expressed in Drosophila melanogaster S2 cells (IL-22Dm) have been grown from polyethylene glycol solutions. To obtain crystals suitable for X-ray diffraction analysis required the separation of different IL-22Dm glycosylation variants and the inclusion of the detergent cetyltrimethylammonium bromide (CTAB) in the crystallization experiments. The crystals belong to space group P2(1), with unit-cell parameters a = 64.88, b = 62.23, c = 139.524 A, beta = 91.35 degrees, and diffract X-rays to 2.6 A resolution. The crystallographic asymmetric unit contains six IL-22Dm molecules, corresponding to a solvent content of approximately 49%.
