Krüger Jan, Sczyrba Alexander, Kurtz Stefan, Giegerich Robert
Technische Fakultät, Universität Bielefeld, D-33594 Bielefeld, Germany.
Nucleic Acids Res. 2004 Jul 1;32(Web Server issue):W301-4. doi: 10.1093/nar/gkh478.
e2g is a web-based server which efficiently maps large expressed sequence tag (EST) and cDNA datasets to genomic DNA. It significantly extends the volume of data that can be mapped in reasonable time, and makes this improved efficiency available as a web service. Our server hosts large collections of EST sequences (e.g. 4.1 million mouse ESTs of 1.87 Gb) in precomputed indexed data structures for efficient sequence comparison. The user can upload a genomic DNA sequence of interest and rapidly compare this to the complete collection of ESTs on the server. This delivers a mapping of the ESTs on the genomic DNA. The e2g web interface provides a graphical overview of the mapping. Alignments of the mapped EST regions with parts of the genomic sequence are visualized. Zooming functions allow the user to interactively explore the results. Mapped sequences can be downloaded for further analysis. e2g is available on the Bielefeld University Bioinformatics Server at http://bibiserv.techfak.uni-bielefeld.de/e2g/.
e2g是一个基于网络的服务器，它能有效地将大量表达序列标签（EST）和cDNA数据集映射到基因组DNA上。它显著扩展了能在合理时间内映射的数据量，并将这种提高的效率作为一项网络服务提供。我们的服务器以预先计算的索引数据结构托管大量EST序列集合（例如，1.87Gb的410万条小鼠EST序列），用于高效的序列比较。用户可以上传感兴趣的基因组DNA序列，并快速将其与服务器上的完整EST集合进行比较。这会生成EST在基因组DNA上的映射。e2g网络界面提供映射的图形化概述。映射的EST区域与基因组序列部分的比对会可视化显示。缩放功能允许用户交互式地探索结果。映射的序列可以下载用于进一步分析。可在比勒费尔德大学的生物信息学服务器上通过http://bibiserv.techfak.uni-bielefeld.de/e2g/访问e2g。
