Min Xiang Jia, Butler Gregory, Storms Reginald, Tsang Adrian
Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec H4B 1R6, Canada.
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W669-72. doi: 10.1093/nar/gki436.
TargetIdentifier is a webserver that identifies full-length cDNA sequences from the expressed sequence tag (EST)-derived contig and singleton data. To accomplish this TargetIdentifier uses BLASTX alignments as a guide to locate protein coding regions and potential start and stop codons. This information is then used to determine whether the EST-derived sequences include their translation start codons. The algorithm also uses the BLASTX output to assign putative functions to the query sequences. The server is available at https://fungalgenome.concordia.ca/tools/TargetIdentifier.html.
TargetIdentifier是一个网络服务器，它从表达序列标签（EST）衍生的重叠群和单拷贝数据中识别全长cDNA序列。为实现这一目标，TargetIdentifier使用BLASTX比对作为指导来定位蛋白质编码区域以及潜在的起始和终止密码子。然后利用这些信息来确定EST衍生序列是否包含其翻译起始密码子。该算法还使用BLASTX输出为查询序列赋予假定的功能。该服务器可在https://fungalgenome.concordia.ca/tools/TargetIdentifier.html获取。
