Min Xiang Jia, Butler Gregory, Storms Reginald, Tsang Adrian
Centre for Structural and Functional Genomics, Concordia University, Montreal, Quebec, Canada H4B 1R6.
Nucleic Acids Res. 2005 Jul 1;33(Web Server issue):W677-80. doi: 10.1093/nar/gki394.
OrfPredictor is a web server designed for identifying protein-coding regions in expressed sequence tag (EST)-derived sequences. For query sequences with a hit in BLASTX, the program predicts the coding regions based on the translation reading frames identified in BLASTX alignments, otherwise, it predicts the most probable coding region based on the intrinsic signals of the query sequences. The output is the predicted peptide sequences in the FASTA format, and a definition line that includes the query ID, the translation reading frame and the nucleotide positions where the coding region begins and ends. OrfPredictor facilitates the annotation of EST-derived sequences, particularly, for large-scale EST projects. OrfPredictor is available at https://fungalgenome.concordia.ca/tools/OrfPredictor.html.
OrfPredictor是一个用于识别来自表达序列标签（EST）序列中的蛋白质编码区域的网络服务器。对于在BLASTX中有匹配结果的查询序列，该程序基于BLASTX比对中确定的翻译阅读框来预测编码区域，否则，则基于查询序列的内在信号来预测最可能的编码区域。输出结果是FASTA格式的预测肽序列，以及一条定义行，其中包括查询ID、翻译阅读框以及编码区域开始和结束的核苷酸位置。OrfPredictor有助于对来自EST的序列进行注释，特别是对于大规模的EST项目。可通过https://fungalgenome.concordia.ca/tools/OrfPredictor.html访问OrfPredictor。
