Lee Bernett T K, Tan Tin Wee, Ranganathan Shoba
Department of Biochemistry, National University of Singapore, 8 Medical Drive, Singapore 117597, Singapore.
Nucleic Acids Res. 2003 Jul 1;31(13):3533-6. doi: 10.1093/nar/gkg561.
Splicing is a biological phenomenon that removes the non-coding sequence from the transcripts to produce a mature transcript suitable for translation. To study this phenomenon, information on the intron-exon arrangement of a gene is essential, usually obtained by aligning mRNA/EST sequences to their cognate genomic sequences. MGAlign is a novel, rapid, memory efficient and practical method for aligning mRNA/EST and genome sequences. We present here a freely available web service, MGAlignIt (http://origin.bic.nus.edu.sg/mgalign/mgalignit), based on MGAlign. Besides the alignment itself, this web service allows users to effectively visualize the alignment in a graphical manner and to perform limited analysis on the alignment output. The server also permits the alignment to be saved in several forms, both graphical and text, suitable for further processing and analysis by other programs.
剪接是一种生物学现象，它从转录本中去除非编码序列，以产生适合翻译的成熟转录本。为了研究这一现象，基因内含子-外显子排列的信息至关重要，通常通过将mRNA/EST序列与其同源基因组序列进行比对来获得。MGAlign是一种用于比对mRNA/EST和基因组序列的新颖、快速、内存高效且实用的方法。我们在此展示了一个基于MGAlign的免费网络服务MGAlignIt（http://origin.bic.nus.edu.sg/mgalign/mgalignit）。除了比对本身，该网络服务还允许用户以图形方式有效地可视化比对结果，并对比对输出进行有限的分析。服务器还允许将比对结果以多种图形和文本形式保存，适合其他程序进行进一步处理和分析。
