Langemann H, Kabiersch A, Newcombe J
Department of Research, Cantonal Hospital Basel, Switzerland.
Eur Neurol. 1992;32(5):248-52. doi: 10.1159/000116835.
The levels of the antioxidants ascorbic acid, cysteine, reduced glutathione and alpha-tocopherol, of the free-radical marker uric acid and of the amino acids tyrosine and tryptophan were measured by means of high-pressure liquid chromatography in plaques, adjacent white matter and distant white matter from patients with multiple sclerosis, and in central nervous system tissue from patients without neurological diseases. Cholesterol and DNA were also determined, to check demyelination and cellularity. Uric acid was increased and glutathione correspondingly decreased in plaques; alpha-tocopherol was lowest in plaques and highest in distant white matter in all cases. Ascorbic acid, cysteine, tyrosine and tryptophan were not significantly changed in any tissue. The results provide evidence supporting the involvement of free radicals in multiple sclerosis.
通过高压液相色谱法测定了多发性硬化症患者斑块、相邻白质和远处白质以及无神经疾病患者中枢神经系统组织中抗氧化剂抗坏血酸、半胱氨酸、还原型谷胱甘肽和α-生育酚、自由基标志物尿酸以及氨基酸酪氨酸和色氨酸的水平。还测定了胆固醇和DNA，以检查脱髓鞘和细胞性。斑块中尿酸增加而谷胱甘肽相应减少；在所有病例中，α-生育酚在斑块中最低，在远处白质中最高。抗坏血酸、半胱氨酸、酪氨酸和色氨酸在任何组织中均无显著变化。这些结果提供了支持自由基参与多发性硬化症的证据。
