Tegenfeldt Jonas O, Prinz Christelle, Cao Han, Chou Steven, Reisner Walter W, Riehn Robert, Wang Yan Mei, Cox Edward C, Sturm James C, Silberzan Pascal, Austin Robert H
Department of Physics, Lund University, SE-221 00 Lund, Sweden.
Proc Natl Acad Sci U S A. 2004 Jul 27;101(30):10979-83. doi: 10.1073/pnas.0403849101. Epub 2004 Jul 13.
We show that genomic-length DNA molecules imaged in nanochannels have an extension along the channel that scales linearly with the contour length of the polymer, in agreement with the scaling arguments developed by de Gennes for self-avoiding confined polymers. This fundamental relationship allows us to measure directly the contour length of single DNA molecules confined in the channels, and the statistical analysis of the dynamics of the polymer in the nanochannel allows us to compute the SD of the mean of the extension. This statistical analysis allows us to measure the extension of lambda DNA multimers with a 130-nm SD in 1 min.
我们表明,在纳米通道中成像的基因组长度DNA分子沿着通道的伸展与聚合物的轮廓长度呈线性比例关系,这与德热纳为自回避受限聚合物所提出的标度论证一致。这种基本关系使我们能够直接测量限制在通道中的单个DNA分子的轮廓长度,并且对纳米通道中聚合物动力学的统计分析使我们能够计算伸展平均值的标准差。这种统计分析使我们能够在1分钟内以130纳米的标准差测量λDNA多聚体的伸展。