Van Trappen Stefanie, Tan Tjhing-Lok, Yang Jifang, Mergaert Joris, Swings Jean
Laboratorium voor Microbiologie, Vakgroep Biochemie, Fysiologie en Microbiologie, Universiteit Gent, K.L. Ledeganckstr. 35, B-9000 Gent, Belgium.
Alfred-Wegener-Institut für Polar- und Meeresforschung, Bremerhaven, Germany.
Int J Syst Evol Microbiol. 2004 Jul;54(Pt 4):1157-1163. doi: 10.1099/ijs.0.02862-0.
Seven novel, cold-adapted, strictly aerobic, facultatively oligotrophic strains, isolated from Antarctic sea water, were investigated by using a polyphasic taxonomic approach. The isolates were Gram-negative, chemoheterotrophic, motile, rod-shaped cells that were psychrotolerant and moderately halophilic. Buds were produced on mother and daughter cells and on prosthecae. Prostheca formation was peritrichous and prosthecae could be branched. Phylogenetic analysis based on 16S rRNA gene sequences indicated that these strains belong to the gamma-Proteobacteria and are related to the genus Alteromonas, with 98.3% sequence similarity to Alteromonas macleodii and 98.0% to Alteromonas marina, their nearest phylogenetic neighbours. Whole-cell fatty acid profiles of the isolates were very similar and included C(16 : 0), C(16 : 1)omega7c, C(17 : 1)omega8c and C(18 : 1)omega8c as the major fatty acid components. These results support the affiliation of these isolates to the genus Alteromonas. DNA-DNA hybridization results and differences in phenotypic characteristics show that the strains represent a novel species with a DNA G+C content of 43-45 mol%. The name Alteromonas stellipolaris sp. nov. is proposed for this novel species; the type strain is ANT 69aT (=LMG 21861T=DSM 15691T). An emended description of the genus Alteromonas is given.
采用多相分类学方法对从南极海水中分离出的7株新型、冷适应、严格需氧、兼性贫营养菌株进行了研究。分离菌株为革兰氏阴性、化能异养、具运动性、杆状细胞，具有耐冷性且为中度嗜盐菌。母细胞、子细胞和菌柄上均可产生芽孢。菌柄形成呈周生，且菌柄可分支。基于16S rRNA基因序列的系统发育分析表明，这些菌株属于γ-变形菌纲，与交替单胞菌属相关，与麦克劳德交替单胞菌的序列相似性为98.3%，与海交替单胞菌的序列相似性为98.0%，它们是最近的系统发育近缘种。分离菌株的全细胞脂肪酸谱非常相似，主要脂肪酸成分包括C(16 : 0)、C(16 : 1)ω7c、C(17 : 1)ω8c和C(18 : 1)ω8c。这些结果支持这些分离菌株属于交替单胞菌属。DNA-DNA杂交结果和表型特征差异表明，这些菌株代表一个新物种，其DNA G+C含量为43 - 45 mol%。为此新物种提议命名为星极交替单胞菌（Alteromonas stellipolaris sp. nov.）；模式菌株为ANT 69aT（=LMG 21861T=DSM 15691T）。并给出了交替单胞菌属的修订描述。
