Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yuseong, Daejon, Republic of Korea.
Department of Food Science and Biotechnology, Sungkyunkwan University, Jangan-gu, Suwon, Republic of Korea.
Int J Syst Evol Microbiol. 2012 Feb;62(Pt 2):347-351. doi: 10.1099/ijs.0.033084-0. Epub 2011 Mar 25.
A Gram-staining-variable, motile, endospore-forming and rod-shaped bacterial strain, IDS-20(T), was isolated from a marine solar saltern in Korea and subjected to a polyphasic taxonomic investigation. Strain IDS-20(T) grew optimally at 37 °C, at pH 7.5-8.0 and in the presence of 4-5 % (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain IDS-20(T) belongs to the genus Virgibacillus. Strain IDS-20(T) exhibited 93.4-96.6 % 16S rRNA gene sequence similarity to the type strains of species of the genus Virgibacillus. Strain IDS-20(T) had MK-7 as the predominant menaquinone and a cell-wall peptidoglycan based on meso-diaminopimelic acid. The major fatty acids were anteiso-C(15 : 0) and anteiso-C(17 : 0) and major polar lipids were diphosphatidylglycerol, phosphatidylglycerol and two unidentified phospholipids. The DNA G+C content was 39.5 mol%. The phylogenetic distinctiveness and differential phenotypic characteristics of strain IDS-20(T) demonstrated that this strain can be distinguished from recognized species of the genus Virgibacillus. On the basis of the data presented, strain IDS-20(T) represents a novel species of the genus Virgibacillus, for which the name Virgibacillus campisalis sp. nov. is proposed. The type strain is IDS-20(T) ( = KCTC 13727(T) = CCUG 59308(T)).
一株革兰氏染色可变、运动、产芽孢的杆状细菌 IDS-20(T) 从韩国的一个海洋太阳盐场中分离出来，并进行了多相分类学研究。菌株 IDS-20(T) 在 37°C、pH7.5-8.0 和 4-5%(w/v)NaCl 的条件下生长最佳。基于 16S rRNA 基因序列的系统发育分析表明，菌株 IDS-20(T) 属于弧菌属。菌株 IDS-20(T)与弧菌属种的模式菌株的 16S rRNA 基因序列相似性为 93.4-96.6%。菌株 IDS-20(T)以 MK-7 作为主要的menaquinone，以 meso-二氨基庚二酸为细胞壁肽聚糖。主要脂肪酸为 anteiso-C(15:0)和 anteiso-C(17:0)，主要极性脂为双磷脂酰甘油、磷脂酰甘油和两种未鉴定的磷脂。DNA G+C 含量为 39.5mol%。菌株 IDS-20(T)的系统发育独特性和差异表型特征表明，该菌株可与弧菌属已识别的种区分开来。基于所提供的数据，菌株 IDS-20(T)代表了弧菌属的一个新种，命名为 campisalis 弧菌属。该模式株为 IDS-20(T)( = KCTC 13727(T) = CCUG 59308(T))。
