Yoon Jung-Hoon, Oh Tae-Kwang, Park Yong-Ha
Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
National Research Laboratory of Molecular Ecosystematics, Institute of Probionic, Probionic Corporation, Bio-venture Center, Korea Research Institute of Bioscience and Biotechnology (KRIBB), PO Box 115, Yusong, Taejon, Korea.
Int J Syst Evol Microbiol. 2004 Nov;54(Pt 6):2163-2167. doi: 10.1099/ijs.0.63196-0.
A Gram-variable, endospore-forming moderately halophilic rod, strain SF-121, was isolated from a marine solar saltern of the Yellow Sea in Korea. The result of 16S rRNA gene sequence analysis showed that strain SF-121 has highest sequence similarity (99.7 %) with the type strain of Bacillus halodenitrificans. Phylogenetic analyses based on 16S rRNA gene sequences revealed that B. halodenitrificans DSM 10037(T) and strain SF-121 are more closely related to the genus Virgibacillus than to the genus Bacillus. Strain SF-121 and B. halodenitrificans DSM 10037(T) exhibited 16S rRNA gene similarity levels of 95.3-97.5 % with the type strains of Virgibacillus species and 94.0 % with the type strain of Bacillus subtilis. DNA-DNA relatedness and phenotypic data indicated that B. halodenitrificans DSM 10037(T) and strain SF-121 are members of the same species. B. halodenitrificans DSM 10037(T) and strain SF-121 exhibited DNA-DNA relatedness values of 9-11 % with the type strains of Virgibacillus carmonensis and Virgibacillus marismortui. On the basis of the phenotypic, chemotaxonomic, phylogenetic and genetic data, B. halodenitrificans should be reclassified in the genus Virgibacillus as Virgibacillus halodenitrificans comb. nov.
从韩国黄海的一个海洋太阳能盐场中分离出一株革兰氏可变、形成芽孢的中度嗜盐杆菌,菌株SF-121。16S rRNA基因序列分析结果表明,菌株SF-121与嗜盐脱氮芽孢杆菌模式菌株的序列相似性最高(99.7%)。基于16S rRNA基因序列的系统发育分析表明,嗜盐脱氮芽孢杆菌DSM 10037(T)和菌株SF-121与 Virgibacillus 属的关系比与芽孢杆菌属的关系更密切。菌株SF-121和嗜盐脱氮芽孢杆菌DSM 10037(T)与 Virgibacillus 属模式菌株的16S rRNA基因相似性水平为95.3-97.5%,与枯草芽孢杆菌模式菌株的相似性水平为94.0%。DNA-DNA相关性和表型数据表明,嗜盐脱氮芽孢杆菌DSM 10037(T)和菌株SF-121是同一物种的成员。嗜盐脱氮芽孢杆菌DSM 10037(T)和菌株SF-121与 Carmonensis Virgibacillus 和 Marismortui Virgibacillus 模式菌株的DNA-DNA相关性值为9-11%。基于表型、化学分类、系统发育和遗传数据,嗜盐脱氮芽孢杆菌应重新分类为 Virgibacillus 属的嗜盐脱氮 Virgibacillus,新组合。