Huang Ying, Cui Qingfeng, Wang Liming, Rodriguez Carlos, Quintana Erika, Goodfellow Michael, Liu Zhiheng
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100080, People's Republic of China.
Antonie Van Leeuwenhoek. 2004 Aug;86(2):159-65. doi: 10.1023/B:ANTO.0000036124.18820.ae.
The taxonomic position of three acidophilic actinomycetes isolated from acidic rhizosphere soil was established using a polyphasic approach. The morphological and chemical properties of the isolates were found to be consistent with their assignment to the genus Streptacidiphilus. Almost complete 16S rRNA gene sequences determined for the strains were aligned with corresponding sequences of representatives of the genera Kitasatospora, Streptacidiphilus and Streptomyces and phylogenetic trees inferred using three tree-making algorithms. The organisms formed a distinct subclade within the Streptacidiphilus 16S rRNA gene tree. They also shared nearly identical phenotypic profiles and rep-PCR fingerprint patterns that readily distinguished them from representatives of the established species of Streptacidiphilus. It is evident from the genotypic and phenotypic data that the three isolates form a new species in the genus Streptacidiphilus. The name proposed for this new species is Streptacidiphilus jiangxiensis, the type strain is isolate 33214T (= AS 4.1857T = JCM 12277T).
采用多相分类方法确定了从酸性根际土壤中分离出的三株嗜酸放线菌的分类地位。发现这些分离株的形态和化学特性与其归入嗜酸链霉菌属相符。为这些菌株测定的几乎完整的16S rRNA基因序列与北里孢菌属、嗜酸链霉菌属和链霉菌属代表的相应序列进行比对，并使用三种建树算法推断系统发育树。这些菌株在嗜酸链霉菌16S rRNA基因树中形成一个独特的亚分支。它们还具有几乎相同的表型特征和rep-PCR指纹图谱，这使它们很容易与嗜酸链霉菌属已确立种的代表区分开来。从基因型和表型数据可以明显看出，这三株分离株在嗜酸链霉菌属中形成了一个新种。这个新种的名称为江西嗜酸链霉菌，模式菌株为分离株33214T（=AS 4.1857T = JCM 12277T）。
