School of Biology, University of Newcastle, Newcastle upon Tyne, NE1 7RU, UK.
Antonie Van Leeuwenhoek. 2013 Aug;104(2):199-206. doi: 10.1007/s10482-013-9938-9. Epub 2013 May 30.
The taxonomic position of three acidophilic actinobacteria, strains FGG38, FGG39 and FSCA67(T), isolated from the fermentation litter layer of a spruce forest soil was established using a polyphasic approach. The strains were shown to have chemotaxonomic and morphological properties consistent with their classification in the genus Streptacidiphilus and formed a distinct phyletic line in the Streptacidiphilus 16S rRNA gene tree being most closely related to Streptacidiphilus albus DSM 41753(T) (99.4 % similarity). DNA:DNA relatedness data showed that isolate FSCA67(T) and the type strain of S. albus belonged to markedly distinct genomic species. The isolates had many phenotypic properties in common and were distinguished readily from their closest phylogenetic neighbours in the Streptacidiphilus gene tree using a broad range of these features. Based on the combined genotypic and phenotypic data the three isolates are considered to represent a new Streptacidiphilus species. The name Streptacidiphilus durhamensis sp. nov. is proposed for this taxon with isolate FSCA67(T) (=DSM 45796(T) = KACC 17154(T) = NCIMB 14828(T)) [corrected] as the type strain.
采用多相分类法确定了从云杉林土壤发酵层分离得到的三株嗜酸放线菌菌株 FGG38、FFG39 和 FSCA67（T）的分类地位。这些菌株的化学分类和形态学特性与它们在 Streptacidiphilus 属中的分类一致，并在 Streptacidiphilus 16S rRNA 基因树中形成了一个独特的系统发育分支，与 Streptacidiphilus albus DSM 41753（T）（相似度为 99.4%）最为密切相关。DNA-DNA 同源性数据表明，FSCA67（T）分离株和 S. albus 的模式株属于明显不同的基因组种。这些分离株具有许多表型特性，并且可以根据这些特征的广泛应用，很容易与 Streptacidiphilus 基因树中最接近的系统发育近邻区分开来。基于综合的基因型和表型数据，这三个分离株被认为代表了一个新的 Streptacidiphilus 种。提议将该分类群命名为 Durham 氏 Streptacidiphilus sp. nov.，以 FSCA67（T）分离株（=DSM 45796（T）=KACC 17154（T）=NCIMB 14828（T））[更正]作为模式株。
