Zhang Jianli, Wu Dongdong, Liu Zhiheng
School of Life Science and Technology, Beijing Institute of Technology, Beijing 100081, PR China.
Int J Syst Evol Microbiol. 2009 May;59(Pt 5):1076-81. doi: 10.1099/ijs.0.65863-0.
An actinomycete isolated from soil from a field of grass was analysed using a polyphasic taxonomic approach. The organism, designated strain W12(T), was found to have a range of chemical and morphological properties consistent with its classification in the genus Saccharopolyspora. An almost-complete 16S rRNA gene sequence determined for the strain was aligned with corresponding sequences for representatives of the genus Saccharopolyspora, using two tree-making algorithms. The novel organism formed a monophyletic clade with the type strain of Saccharopolyspora antimicrobica, but the DNA-DNA relatedness value was low. Strain W12(T) also had a phenotypic profile that readily distinguished it from recognized representatives of the genus Saccharopolyspora. It is evident from the combined genotypic and phenotypic data that the new organism should be classified as a novel species of the genus Saccharopolyspora. The name proposed for this new taxon is Saccharopolyspora jiangxiensis sp. nov., with strain W12(T) (=CGMCC 4.3529(T)=JCM 14613(T)) as the type strain.
采用多相分类方法对从草地土壤中分离出的一株放线菌进行了分析。该菌株命名为W12(T)，发现其具有一系列化学和形态学特性，与其在糖多孢菌属中的分类相符。使用两种建树算法，将该菌株测定的近乎完整的16S rRNA基因序列与糖多孢菌属代表菌株的相应序列进行比对。该新菌株与抗微生物糖多孢菌模式菌株形成一个单系分支，但DNA-DNA杂交值较低。W12(T)菌株还具有易于与糖多孢菌属已确认代表菌株区分的表型特征。综合基因型和表型数据表明，该新菌株应归类为糖多孢菌属的一个新物种。提议的这个新分类单元的名称是江西糖多孢菌（Saccharopolyspora jiangxiensis sp. nov.），模式菌株为W12(T)（=CGMCC 4.3529(T)=JCM 14613(T)）。
