来自甲基营养型假单胞菌的HMG-CoA还原酶的结晶。
Crystallization of HMG-CoA reductase from Pseudomonas mevalonii.
作者信息
Lawrence C M, Chi Y I, Rodwell V W, Stauffacher C V
机构信息
Department of Biological Sciences, Purdue University, West Lafayette, Indiana 47907, USA.
出版信息
Acta Crystallogr D Biol Crystallogr. 1995 May 1;51(Pt 3):386-9. doi: 10.1107/S0907444994009819.
Crystals of 3-hydroxy-3-methylglutaryl coenzyme A (HMG-CoA) reductase from Pseudomonas mevalonii have been grown by vapor diffusion in hanging drops at pH 6.7 using ammonium sulfate as the precipitant. Serial dilution seeding and manipulation of glycerol concentration were both used to obtain crystals larger than 1.0 mm. The crystals are cubic, space group I4(1)32, with a = 229.4 A. A V(m) value of 2.71 A(3) Da(-l) indicates 96 molecules per unit cell with two molecules in the asymmetric unit. These crystals diffract to 2.8 A with conventional X-ray sources, and beyond 2.4 A with synchrotron radiation.
利用硫酸铵作为沉淀剂，通过悬滴气相扩散法，在pH 6.7条件下培养出了来自甲基营养型假单胞菌的3-羟基-3-甲基戊二酰辅酶A（HMG-CoA）还原酶晶体。采用连续稀释接种法和调节甘油浓度的方法，获得了尺寸大于1.0毫米的晶体。这些晶体呈立方晶型，空间群为I4(1)32，a = 229.4 Å。V(m)值为2.71 ų Da⁻¹，表明每个晶胞中有96个分子，不对称单元中有两个分子。这些晶体在传统X射线源下可衍射至2.8 Å，在同步辐射下可衍射至2.4 Å以上。
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