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人类免疫缺陷病毒（HIV）受体CD4和共受体CCR5在活细胞中的流动性：对HIV融合和进入事件的影响。

Mobility of the human immunodeficiency virus (HIV) receptor CD4 and coreceptor CCR5 in living cells: implications for HIV fusion and entry events.

作者信息

Steffens Carolyn M, Hope Thomas J

机构信息

Department of Microbiology and Immunology, University of Illinois at Chicago, Chicago, Illinois, USA.

出版信息

J Virol. 2004 Sep;78(17):9573-8. doi: 10.1128/JVI.78.17.9573-9578.2004.

DOI:10.1128/JVI.78.17.9573-9578.2004
PMID:15308751
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC506925/
Abstract

The sequence of events leading to human immunodeficiency virus fusion and entry likely involves the recruitment of multiple receptor and coreceptor proteins to a specific complex by the viral envelope. Using fluorescence recovery after photobleaching technology, we find that both CD4 and CCR5 are mobile in the cell membrane. Interestingly, our findings also suggest that the seven-span transmembrane coreceptor is significantly more mobile than CD4 and requires membrane cholesterol for mobility.

摘要

导致人类免疫缺陷病毒融合和进入的一系列事件可能涉及病毒包膜将多种受体和共受体蛋白招募到特定复合物中。利用光漂白后荧光恢复技术，我们发现CD4和CCR5在细胞膜中都是可移动的。有趣的是，我们的研究结果还表明，七跨膜共受体的移动性明显高于CD4，并且其移动性需要膜胆固醇。

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本文引用的文献

1
Localization of CD4 and CCR5 in living cells.CD4和CCR5在活细胞中的定位。
J Virol. 2003 Apr;77(8):4985-91. doi: 10.1128/jvi.77.8.4985-4991.2003.
2
Role of cholesterol in human immunodeficiency virus type 1 envelope protein-mediated fusion with host cells.胆固醇在1型人类免疫缺陷病毒包膜蛋白介导的与宿主细胞融合中的作用。
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Blocking of HIV-1 infection by targeting CD4 to nonraft membrane domains.通过将CD4靶向非脂筏膜结构域来阻断HIV-1感染。
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Cholesterol is essential for macrophage inflammatory protein 1 beta binding and conformational integrity of CC chemokine receptor 5.胆固醇对于巨噬细胞炎性蛋白1β的结合以及CC趋化因子受体5的构象完整性至关重要。
Blood. 2002 Jun 15;99(12):4298-306. doi: 10.1182/blood-2001-11-0087.
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Human immunodeficiency virus type 1 uses lipid raft-colocalized CD4 and chemokine receptors for productive entry into CD4(+) T cells.1型人类免疫缺陷病毒利用脂筏共定位的CD4和趋化因子受体有效进入CD4(+) T细胞。
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p56Lck anchors CD4 to distinct microdomains on microvilli.p56Lck将CD4锚定到微绒毛上不同的微结构域。
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Association of chemokine-mediated block to HIV entry with coreceptor internalization.趋化因子介导的对HIV进入的阻断与共受体内化的关联。
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Lipid rafts and HIV pathogenesis: host membrane cholesterol is required for infection by HIV type 1.脂筏与HIV发病机制：宿主细胞膜胆固醇是1型HIV感染所必需的。
AIDS Res Hum Retroviruses. 2001 Jul 20;17(11):1009-19. doi: 10.1089/088922201300343690.
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From fixed to FRAP: measuring protein mobility and activity in living cells.从固定到荧光恢复后光漂白：测量活细胞中的蛋白质流动性和活性
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Membrane raft microdomains mediate lateral assemblies required for HIV-1 infection.膜筏微结构域介导HIV-1感染所需的侧向组装。
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