小鼠巨噬细胞中的胞质pH调节。质膜定位的H(+)-ATP酶介导的质子外排。
Cytosolic pH regulation in mouse macrophages. Proton extrusion by plasma-membrane-localized H(+)-ATPase.
作者信息
Tapper H, Sundler R
机构信息
Department of Medical and Physiological Chemistry, Lund University, Sweden.
出版信息
Biochem J. 1992 Jan 1;281 ( Pt 1)(Pt 1):245-50. doi: 10.1042/bj2810245.
Abstract
Recent evidence indicates that H+ extrusion in macrophages is in part accomplished by a H(+)-ATPase of vacuolar type. The presence and plasma-membrane localization of such a mechanism in adherent resident macrophages was verified by inhibition of H+ extrusion, monitored by changes in both cytosolic pH (pHi) and extracellular pH, with low concentrations of the H(+)-ATPase inhibitors N-ethylmaleimide and 7-chloro-4-nitrobenz-2-oxa-1,3-diazole. The H(+)-ATPase was operative at physiological pHi levels, thus contributing to maintenance of steady-state pHi. It was further shown to be sensitive to the plasma-membrane potential, with hyperpolarization being strongly inhibitory. In addition, H+ extrusion mediated by the H(+)-ATPase and the generation and release of lactic acid caused acidification of the pericellular space and could enable secreted lysosomal hydrolases to act extracellularly.
摘要
最近的证据表明，巨噬细胞中的H⁺排出部分是由液泡型H⁺-ATP酶完成的。通过用低浓度的H⁺-ATP酶抑制剂N-乙基马来酰亚胺和7-氯-4-硝基苯并-2-恶唑-1,3-二唑抑制H⁺排出（通过胞质pH（pHi）和细胞外pH的变化进行监测），证实了这种机制在贴壁驻留巨噬细胞中的存在及其质膜定位。H⁺-ATP酶在生理pHi水平下起作用，从而有助于维持稳态pHi。进一步表明它对质膜电位敏感，超极化具有强烈的抑制作用。此外，由H⁺-ATP酶介导的H⁺排出以及乳酸的产生和释放导致细胞周隙酸化，并可使分泌的溶酶体水解酶在细胞外发挥作用。
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本文引用的文献
1
AN IMPROVED PROCEDURE FOR THE PREPARATION OF CRYSTALLINE LACTIC DEHYDROGENASE FROM HOG HEART.一种从猪心制备结晶乳酸脱氢酶的改进方法。
J Biol Chem. 1963 Dec;238:3853-8.
2
Lactic acid assay with L(plus)lactic acid dehydrogenase from rabbit muscle.用兔肌肉来源的L(+)乳酸脱氢酶进行乳酸测定。
Acta Physiol Scand. 1963 Jun-Jul;58:243-9. doi: 10.1111/j.1748-1716.1963.tb02645.x.
3
The reactivity of SH groups with a fluorogenic reagent.巯基与一种荧光试剂的反应活性。
FEBS Lett. 1970 Feb 25;6(4):346-348. doi: 10.1016/0014-5793(70)80095-3.
4
Intracellular pH.细胞内pH值
Physiol Rev. 1981 Apr;61(2):296-434. doi: 10.1152/physrev.1981.61.2.296.
5
beta-Lactamase activity in human pus.人脓液中的β-内酰胺酶活性
J Infect Dis. 1980 Oct;142(4):594-601. doi: 10.1093/infdis/142.4.594.
6
Transport of protons across membranes by weak acids.弱酸介导的质子跨膜转运
Physiol Rev. 1980 Jul;60(3):825-63. doi: 10.1152/physrev.1980.60.3.825.
7
Cell-mediated extracellular acidification and bone resorption: evidence for a low pH in resorbing lacunae and localization of a 100-kD lysosomal membrane protein at the osteoclast ruffled border.细胞介导的细胞外酸化与骨吸收：骨吸收陷窝低pH值的证据以及一种100-kD溶酶体膜蛋白在破骨细胞皱褶缘的定位
J Cell Biol. 1985 Dec;101(6):2210-22. doi: 10.1083/jcb.101.6.2210.
8
Rates of utilization and fates of glucose, glutamine, pyruvate, fatty acids and ketone bodies by mouse macrophages.小鼠巨噬细胞对葡萄糖、谷氨酰胺、丙酮酸、脂肪酸和酮体的利用速率及去向
Biochem J. 1987 Mar 15;242(3):631-6. doi: 10.1042/bj2420631.
9
Isolated osteoclasts resorb the organic and inorganic components of bone.分离的破骨细胞会吸收骨骼的有机和无机成分。
J Cell Biol. 1986 Apr;102(4):1164-72. doi: 10.1083/jcb.102.4.1164.
10
Pus potassium.脓液钾。
Inflammation. 1988 Feb;12(1):37-43. doi: 10.1007/BF00915890.
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