寻常型银屑病患者中CC趋化因子配体20和CC趋化因子受体6信使核糖核酸的表达
Expression of CC chemokine ligand 20 and CC chemokine receptor 6 mRNA in patients with psoriasis vulgaris.
作者信息
Wu Yan, Li Jiawen
机构信息
Department of Dermatology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430022, China.
出版信息
J Huazhong Univ Sci Technolog Med Sci. 2004;24(3):297-9. doi: 10.1007/BF02832019.
In order to explore the possible role of CC chemokine ligand 20 (CCL20) and its receptor CC chemokine receptor 6 (CCR6) in the pathogenesis of psoriasis, the expression levels of mRNA of them in psoriatic lesions were investigated. The skin biopsies were collected from skin lesions in 35 cases of psoriasis vulgaris and 18 normal controls. RT-PCR was used to semi-quantitatively analyze the mRNA expression of CCL20 and CCR6 in the psoriatic lesions and the normal skin tissues. The results showed that the mRNA of CCL20 and CCR6 was present in every specimen. The expression levels of CCL20 mRNA in skin lesions were 1.1397 +/- 0.0521, which were greatly higher than those in normal controls (0.8681 +/- 0.0308) (P<0.001). The expression levels of CCR6 mRNA in skin lesions were 1.1103 +/- 0.0538, significantly higher than in the controls (0.9131 +/- 0.0433, P<0.001). These findings indicate that up-regulated expression of CCL20 and CCR6 mRNA might be related to the pathogenesis of psoriasis.
为探讨CC趋化因子配体20（CCL20）及其受体CC趋化因子受体6（CCR6）在银屑病发病机制中的可能作用，研究了它们在银屑病皮损中的mRNA表达水平。收集35例寻常型银屑病患者皮损及18例正常对照者的皮肤活检组织。采用逆转录聚合酶链反应（RT-PCR）半定量分析银屑病皮损及正常皮肤组织中CCL20和CCR6的mRNA表达。结果显示，每个标本中均存在CCL20和CCR6的mRNA。皮损中CCL20 mRNA表达水平为1.1397±0.0521，显著高于正常对照（0.8681±0.0308）（P<0.001）。皮损中CCR6 mRNA表达水平为1.1103±0.0538，显著高于对照（0.9131±0.0433，P<0.001）。这些结果表明，CCL20和CCR6 mRNA表达上调可能与银屑病的发病机制有关。
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