Zhang Jian, Lakowicz Joseph R
Center for Fluorescence Spectroscopy, University of Maryland School of Medicine, Department of Biochemistry, 725 West Lombard Street, Baltimore, Maryland 21201, USA.
J Phys Chem B. 2005 May 12;109(18):8701-6. doi: 10.1021/jp046016j.
Tiopronin-coated silver particles (average diameters of core = 1.6 nm) were prepared by a modified Brust method, and the ligands on the metal core were partially displaced by (2-mercapto-propionylamino) acetic acid-2,5-dioxo-pyrrolidin-1-ylester through ligand exchanges. The particles were bound on amine-pendent polymer backbones by condensation to generate compact aggregates of particles. The aggregated particles displayed a plasmon absorbance rising at 440 nm. Luminescence was enhanced to about 2 times with increasing the plasmon rising by the particle aggregation upon excitation at 400 nm when the polymer was labeled by 3,8-diamino-6-phenyl-phenanthridine.
通过改良的布斯特方法制备了硫普罗宁包覆的银颗粒（核心平均直径 = 1.6 纳米），金属核心上的配体通过配体交换被（2-巯基丙酰氨基）乙酸-2,5-二氧代吡咯烷-1-基酯部分取代。通过缩合将颗粒结合在胺基聚合物主链上，以生成紧密的颗粒聚集体。聚集的颗粒在 440 纳米处显示出等离子体吸收增加。当聚合物用 3,8-二氨基-6-苯基菲啶标记时，在 400 纳米激发下，随着颗粒聚集导致的等离子体增加，发光增强至约 2 倍。
