Chen Yen-Meng, Chung Ying-Chien, Wang Li-Woan, Chen Kung-Tung, Li Shyh-Yuan
Department of Industrial Engineering and Management, China Institute of Technology, Taipei City, Taiwan 115, R.O.C.
J Environ Sci Health A Tox Hazard Subst Environ Eng. 2002 Aug;37(7):1379-90. doi: 10.1081/ese-120005993.
The antimicrobial properties of chitosan, a derivative of chitin, were investigated in the solid and liquid culture against bacteria associated with waterborne disease in order to assess the potential for using chitosan as a natural disinfectant. Six strains which included three gram-negative and three gram-positive bacteria were studied. The effects of the deacetylation degree, concentration, and molecular weight of chitosan on antibacterial activities were assessed. Chitosan exhibited the highest antibacterial activity against the Pseudomonas aeruginosa on the solid agar. Similar tendency was found when the bacteria were cultivated in liquid broth. The higher deacetylation degree and higher concentration of chitosan cause the higher antibacterial activities. The effect of molecular weight of chitosan on the inhibition efficacy of bacteria is dependent on the species of bacteria. Escherichia coli is sensitive to chitosan during its death phase and logarithmic phase. The antibacterial mechanism of chitosan was illustrated by the surface charge and persistence length. Results indicated that chitosan is potential as a natural disinfectant.
壳聚糖是几丁质的衍生物，为评估其作为天然消毒剂的潜力，对其在固体和液体培养中针对与水传播疾病相关细菌的抗菌特性进行了研究。研究了六种菌株，包括三种革兰氏阴性菌和三种革兰氏阳性菌。评估了壳聚糖的脱乙酰度、浓度和分子量对抗菌活性的影响。壳聚糖在固体琼脂上对铜绿假单胞菌表现出最高的抗菌活性。当细菌在液体肉汤中培养时也发现了类似趋势。壳聚糖的脱乙酰度越高、浓度越高，抗菌活性越高。壳聚糖分子量对细菌抑制效果的影响取决于细菌种类。大肠杆菌在死亡期和对数期对壳聚糖敏感。通过表面电荷和持久长度阐述了壳聚糖的抗菌机制。结果表明壳聚糖有作为天然消毒剂的潜力。
